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First cultivation and characterization of mycobacterium ulcerans from the envir...
Portaels, Françoise; Meyers, Wayne M.; Ablordey, Anthony; Castro, António G.; Chemlal, Karim; Rijk, Pim de; Elsen, Pierre; Fissette, KristaBackground: Mycobacterium ulcerans disease, or Buruli ulcer (BU), is an indolent, necrotizing infection of skin, subcutaneous tissue and, occasionally, bones. It is the third most common human mycobacteriosis worldwide, after tuberculosis and leprosy. There is evidence that M. ulcerans is an environmental pathogen transmitted to humans from aquatic niches; however, well-characterized pure cultures of M. ulceran...
GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Vale...
Azevedo,Fernando Alves de; Mourão Filho,Francisco de Assis Alves; Schinor,Evandro Henrique; Paoli,Luis Gustavo de; Mendes,Beatriz Madalena JanuzziThe objective of this work was the transformation of tobacco and 'Valencia' sweet orange with the GUS gene driven by the citrus phenylalanine ammonia-lyase (PAL) gene promoter (CsPP). Transformation was accomplished by co-cultivation of tobacco and 'Valência' sweet orange explants with Agrobacterium tumefaciens containing the binary vector CsPP-GUS/2201. After plant transformation and regeneration, histochemica...
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