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Separation of human immunoglobulin G subclasses using monolithic column
Leblebici, P.; Rodrigues, A.E.; Pais, L.S.The present study aims to separate hIgG into its subclasses using monolithic stationary phase.
Data: 2013
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Origem: Biblioteca Digital do IPB
Monolith technology in isolation of human immunoglobulin G subclasses
Leblebici, P.; Rodrigues, A.E.; Pais, L.S.In the present study, a method was developed for the separation of hIgG into its subclasses with the advantages of monolithic technology. pH elution was applied using Protein A monolith column in a stepwise method and conditions were optimized. Since IgG3 has very low affinity to Protein A, elution was occurred immediately upon injection of the sample. IgG1 and IgG4 were eluted simultaneously under the applied ...
Data: 2013
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Origem: Biblioteca Digital do IPB
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