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In vitro Multiplication of Aromatic and Medicinal Plants and Fungicide Activity
Leal, Fernanda; Matos, Manuela; Coelho, Ana Cláudia; Carnide, OlindaAromatic and medicinal plants, widely used as folk medicine are, beyond fruits, vegetables grains and spices, the principal source of antioxidant compounds. Several studies demonstrated that antioxidants have also antifungal activity (Jayashree & Subramanyam, 2000; Rasooli & Abyaneh, 2004). More and more, humanity try to replace synthetic metabolites by natural metabolites. Therefore, studies in aromatic and me...
Development and analytical characterization of ofloxacin ion-selective electrodes
Souto, Renata; Pimenta, Adriana; Leal, Fernanda; Catarino, Rita; Santos, Gory; Tavares, TâniaAn ofloxacin-selective electrode based on incorporation of a ofloxacin-tetrakis[3,5- bis(trifluoromethyl)phenyl]borate ion-pair complex in a poly(vinyl chloride) membrane was developed. The sensor displayed a Nernstian response over a wide concentration range (7×10-6–1×10-2 mol L-1) with a slope of 54.9 ± 1.3 mV per decade of concentration, a detection limit of 4×10-6 mol L-1 and adequate selectivity toward som...
Incorrect DNA methylation of the DAZL promoter CpG island associates with defec...
Navarro-Costa, Paulo; Nogueira, Paulo; Carvalho, Marta; Leal, Fernanda; Cordeiro, Inês; Calhaz-Jorge, Carlos; Gonçalves, João; Plancha, Carlos E.Versão impressa: Hum Reprod. 2010 Oct;25(10):2647-54 ; Background: Successful gametogenesis requires the establishment of an appropriate epigenetic state in developing germ cells. Nevertheless, an association between abnormal spermatogenesis and epigenetic disturbances in germline-specific genes remains to be demonstrated. Methods: In this study, the DNA methylation pattern of the promoter CpG island (CGI) of ...
Importância da especiação de metais na avaliação do seu impacto na saúde humana...
Leal, Fernanda; Catarino, Rita; Pimenta, Adriana; Souto, RenataA voltametria de redissolução com eléctrodos de filme de mercúrio (mFes) constitui uma ferramenta poderosa para a monitorização de metais vestigiais. recentemente, os eléctrodos de filme de bismuto (bFes) apareceram como uma alternativa vantajosa, apresentando uma toxicidade negligenciável para o ambiente. neste trabalho são propostas duas novas metodologias (em batch e em fluxo), que se baseiam na realização d...
Phlebotomine sand flies (Diptera, Psychodidae) in an American tegumentary leish...
Virgens,Thieres Marassati das; Santos,Claudiney Biral dos; Pinto,Israel de Souza; Silva,Kleber Silveira da; Leal,Fernanda Cristina; Falqueto,AloísioAmerican tegumentary leishmaniasis is endemic to the Espírito Santo State, Brazil, where it is widely distributed. The composition of the phlebotomine sand fly fauna in an American tegumentary leishmaniasis focus was determined by monthly sampling, using Shannon light traps in an Atlantic Forest reserve and adjacent habitat that had been modified by human activity. Seasonal fluctuations in numbers of the most a...
Capacidade antioxidante de hortaliças usualmente consumidas
Melo,Enayde de Almeida; Maciel,Maria Inês Sucupira; Lima,Vera Lúcia Arroxelas Galvão; Leal,Fernanda Lídia Lemos; Caetano,Ana Carla da SilvaComo objetivo de avaliar a capacidade antioxidante de 15 hortaliças comercializadas na Cidade do Recife, extratos metanólicos foram testados quanto a atividade antioxidante em sistema modelo beta-caroteno/ácido linoléico e a habilidade de seqüestrar o radical estável 1,1-difenil-2-picrilhidrazil (DPPH). Todas as hortaliças investigadas exibiram propriedade antioxidante, entretanto a ação foi diferenciada entre ...
Characterization of grapevine (Vitis vinifera L.) cultivars from northern Portu...
Pinto-Carnide, Olinda; Martín, Juan Pedro; Leal, Fernanda; Castro, Isaura; Guedes-Pinto, Henrique; Oriz, Jesús MaríaTwelve grapevine cultivars from northern Portugal were characterized with RAPD and microsatellites. Nine primers were used in the RAPD analysis; 8 of the varieties showed monotypic patterns. With the 6 microsatellite loci a total of 38 alleles was obtained. Relationships among the studied varieties were observed. Both types of molecular markers have proved useful for identification. Existence of synonymies is d...
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