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Image analysis for automatic characterization of polyhydroxyalcanoates granules
Mesquita, D. P.; Selvaggio, Gianluca; Cunha, J. R.; Leal, Cristiano S.; Amaral, A. L.; Ferreira, E. C.A new monitoring approach for polyhydroxyalcanoates (PHA) granules identification and characterization based on image analysis procedures is proposed. PHA granules were analyzed by Sudan Black B (SBB) staining in an enhanced biological phosphorus removal (EBPR) system. Color images captured on an optical microscope were analyzed through quantitative image analysis. The distribution of PHA granules was estimated...
Novel method to quantify intracellular accumulation of polyphosphate in EBPR sy...
Mesquita, D. P.; Amaral, A. L.; Leal, Cristiano; Carvalheira, Mónica; Cunha, J. R.; Oehmen, Adrian; Reis, Maria A. M.; Ferreira, E. C.A new method for intracellular storage polyphosphate (poly-P)identification and quantification in enhanced biological phosphorus removal (EBPR) systems is proposed based on image analysis. In EBPR systems, 4',6-diamidino-2-phenylindole (DAPI) is usually combined with fluororescent in situ hybridization (FISH) to evaluate the microbial community. The proposed technique is based on an image analysis procedure spe...
Biopolymer monitoring using quantitative image analysis techniques
Carvalheira, Mónica; Mesquita, D. P.; Leal, Cristiano; Oehmen, Adrian; Carvalho, Gilda; Cunha, J. R.; Amaral, A. L.; Ferreira, E. C.; Reis, Maria A. M.Polyhydroxyalkanoates (PHAs) are intracellular biopolymers with many applications, particularly as substitutes of polypropylene and polyethylene, due to their thermoplastic properties and biocompatible nature. Furthermore, glycogen is a polysaccharide of glucose with high importance in the metabolism of microbial communities and polyphosphate is a microbial storage compound that should be recovered in order to ...
Desenvolvimento de metodologias de análise de imagem para quantificar PHA, poli...
Amaral, A. L.; Leal, Cristiano S.; Mesquita, D. P.; Cunha, J. R.; Oehmen, Adrian; Reis, Maria A. M.; Ferreira, E. C.O processo de remoção biológica de fósforo, em estações de tratamento de águas residuais, é um processo efetuado por culturas mistas contendo organismos acumuladores de polifosfatos (PAO) e de glicogénio (GAO). No decurso deste processo os microrganismos podem formar inclusões de glicogénio, polihidroxialcanoatos (PHA) e polifosfatos (poli-P). Neste processo, é fulcral monitorizar o metabolismo intracelular par...
Polyphosphates and poly-β-hydroxybutyrate granules identification through quant...
Mesquita, D. P.; Leal, Cristiano; Oehmen, Adrian; Amaral, A. L.; Reis, Maria A. M.; Ferreira, E. C.Enhanced biological phosphorus removal (EBPR) is a widely implemented technique for having the potential to cheaply and reliably remove phosphate from wastewater treatment processes, than traditional chemical methods. EBPR is performed by operating the system sequentially with anaerobic and aerobic conditions. Several studies were already performed ranging from different strategies for the competition between p...
O impacto emocional da cirurgia bariátrica em pacientes com obesidade mórbida
Leal,Cristiano Waihrich; Baldin,NelmaA cirurgia bariátrica é um método que tem sido utilizado para o emagrecimento e resgate da saúde. Em muitos casos, porém, o emagrecimento súbito incorre em quadros psiquiátricos, tais como: sintomas depressivos, ansiedade, uso de substâncias, alterações comportamentais e ideação suicida, dentre outras situações, associadas às mudanças emocionais que o paciente vivencia com o novo estado físico e psíquico. O atu...
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