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Comparative transcriptome analysis between original and evolved recombinant lac...
Guimarães, Pedro M. R.; Le Berre, V.; Sokol, Serguei; François, J.; Teixeira, J. A.; Domingues, LucíliaThe engineering of Saccharomyces cerevisiae strains for lactose utilization has been attempted with the intent of developing high productivity processes for alcoholic fermentation of cheese whey. A recombinant S. cerevisiae flocculent strain that efficiently ferments lactose to ethanol was previously obtained by evolutionary engineering of an original recombinant that displayed poor lactose fermentation perform...
Adaptive evolution of a recombinant lactose-consuming Saccharomyces cerevisiae ...
Guimarães, Pedro M. R.; Parrou, J. L.; Le Berre, V.; François, J.; Teixeira, J. A.; Domingues, LucíliaIn previous work, a recombinant S. cerevisiae flocculent strain (NCYC869-A3/T1, or simply T1) with the ability to express both the LAC4 (coding for beta-galactosidase) and LAC12 (lactose permease) genes of Kluyveromyces lactis was constructed (Domingues et al., Appl Microbiol Biotechnol 51:621–626, 1999). The original recombinant obtained (T1) was able to metabolise lactose but slowly. Thus, it was subjected to...
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