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Genome-wide identification of genes involved in the positive and negative regul...

Background: Acetic acid is mostly known as a toxic by-product of alcoholic fermentation carried out by Saccharomyces cerevisiae, which it frequently impairs. The more recent finding that acetic acid triggers apoptotic programmed cell death (PCD) in yeast sparked an interest to develop strategies to modulate this process, to improve several biotechnological applications, but also for biomedical research. Indeed,...

Ammonium-dependent shortening of CLS in yeast cells starved for essential amino...

Ammonium (NH4(+)) leads to chronological life span (CLS) shortening in Saccharomyces cerevisiae BY4742 cells, particularly evident in cells starved for auxotrophy-complementing amino acids (leucine, lysine, and histidine) simultaneously. Here, we report that the effect of NH4(+) on aging yeast depends on the specific amino acid they are deprived of. Compared with no amino acid starvation, starvation for leucine...

The genome sequence of the highly acetic acid-tolerant zygosaccharomyces bailii...

In this work, it is described the sequencing and annotation of the genome of the yeast strain ISA1307, isolated from a sparkling wine continuous production plant. This strain, formerly considered of the Zygosaccharomyces bailii species, has been used to study Z. bailii physiology, in particular, its extreme tolerance to acetic acid stress at low pH. The analysis of the genome sequence described in this work ind...

Growth culture conditions and nutrient signaling modulating yeast chronological...

The manipulation of nutrient-signaling pathways in yeast has uncovered the impact of environmental growth conditions in longevity. Studies using calorie restriction show that reducing glucose concentration of the culture media is sufficient to increase replicative and chronological lifespan (CLS). Other components of the culture media and factors such as the products of fermentation have also been implicated in...

Ammonium Is toxic for aging yeast cells, inducing death and shortening of the c...

Here we show that in aging Saccharomyces cerevisiae (budding yeast) cells, NH4+ induces cell death associated with shortening of chronological life span. This effect is positively correlated with the concentration of NH4+ added to the culture medium and is particularly evident when cells are starved for auxotrophy-complementing amino acids. NH4+-induced cell death is accompanied by an initial small increase of ...

The fate of acetic acid during glucose co-metabolism by the spoilage yeast Zygo...

Zygosaccharomyces bailii is one of the most widely represented spoilage yeast species, being able to metabolise acetic acid in the presence of glucose. To clarify whether simultaneous utilisation of the two substrates affects growth efficiency, we examined growth in single- and mixed-substrate cultures with glucose and acetic acid. Our findings indicate that the biomass yield in the first phase of growth is the...

Ammonium blocks chronological lifespan extension of extreme calorie restriction...

We have previously shown that ammonium stimulates cell death in amino acid-deprived auxotrophic cells of Saccharomyces cerevisiae (aa-starved cells) transferred to water, whereas the same cells in absence of ammonium displayed chronological lifespan (CLS) expansion typical of extreme starvation conditions. The enhanced loss of cell viability in the presence of ammonium was accompanied by an initial small increa...

Caloric restriction or catalase inactivation extends yeast chronological lifesp...

The free radical theory of aging posits oxidative damage to macromolecules as a primary determinant of lifespan. Recent studies challenge this theory by demonstrating that in some cases, longevity is enhanced by inactivation of oxidative stress defenses or is correlated with increased, rather than decreased reactive oxygen species and oxidative damage. Here we show that, in Saccharomyces cerevisiae, caloric res...

The production of hydrogen sulphide and other aroma compounds by wine strains o...

Genome size and ploidy of Paracoccidioides brasiliensis reveals a haploid DNA c...

The aim of this study was to evaluate genome size and ploidy of the dimorphic pathogenic fungus Paracoccidioides brasiliensis. The cell cycle analysis of 10 P. brasiliensis isolates by flow cytometry (FCM) revealed a genome size ranging from 26.3+/-0.1Mb (26.9+/-0.1fg) to 35.5+/-0.2Mb (36.3+/-0.2fg) per uninucleated yeast cell. The DNA content of conidia from P. brasiliensis ATCC 60855-30.2+/-0.8Mb (30.9+/-0.8f...