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Biochemical and spectroscopic characterization of the membrane-bound nitrate re...
Correia, Cristina; Besson, Stéphane; Brondino, Carlos D.; González, Pablo J.; Lampreia, Jorge; Moura, Isabel; Moura, José J. G.J Biol Inorg Chem (2008) 13:1321–1333 DOI 10.1007/s00775-008-0416-1 ; Membrane-bound nitrate reductase from Marinobacter hydrocarbonoclasticus 617 can be solubilized in either of two ways that will ultimately determine the presence or absence of the small (Iota) subunit. The enzyme complex (NarGHI) is composed of three subunits with molecular masses of 130, 65, and 20 kDa. This enzyme contains approximately 14...
The isolation and characterization of cytochrome c nitrite reductase subunits (...
Almeida, Maria Gabriela; Macieira, Sofia; Gonçalves, Luisa L.; Romão, Maria J.; Costa, Cristina; Lampreia, Jorge; Moura, José J. G.; Moura, IsabelEur. J. Biochem. 270, 3904–3915 (2003) doi:10.1046/j.1432-1033.2003.03772.x ; The cytochrome c nitrite reductase is isolated from the membranes of the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 as a heterooligomeric complex composed by two subunits (61 kDa and 19 kDa) containing c-type hemes, encoded by the genes nrfA and nrfH, respectively. The extracted complex has in average a 2NrfA:1...
The isolation and characterization of cytochrome c nitrite reductase subunits (...
Moura, Isabel; Almeida, Maria Gabriela; Macieira, Sofia; Gonçalves, Luisa L.; Huber, Robert; Cunha, Carlos A.; Romão, Maria J.; Costa, CristinaEur. J. Biochem. 270, 3904–3915 (2003) ; The cytochrome c nitrite reductase is isolated from the membranes of the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 as a heterooligomeric complex composed by two subunits (61 kDa and 19 kDa) containing c-type hemes, encoded by the genes nrfA and nrfH,respectively. The extracted complex has in average a 2NrfA:1NrfH composition. The separation of cc...
Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774
Moura, Isabel; Cunha, Carlos A.; Macieira, Sofia; Dias, João M.; Almeida, Gabriela; Gonçalves, Luisa L.; Costa, Cristina; Lampreia, Jorge; Huber, RobertThe Journal of Biological Chemistry Vol. 278, No. 19, Issue of May 9, pp. 17455–17465, 2003 ; The gene encoding cytochrome c nitrite reductase(NrfA) from Desulfovibrio desulfuricans ATCC 27774 was sequenced and the crystal structure of the enzyme was determined to 2.3-Å resolution. In comparison with homologous structures, it presents structural differences mainly located at the regions surrounding the putativ...
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