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Fps1p channel is the mediator of the major part of glycerol passive diffusion i...
Oliveira, Rui Pedro Soares de; Lages, Fernanda; Graça, Magda Maria Couto da Silva; Lucas, CândidaGlycerol has been shown to cross Saccharomyces cerevisiae plasma membrane (1) through a H+/symport detected in cells grown on non-fermentable carbon sources, (2) by passive diffusion and (3) through the constitutive Fps1p channel. This has been named a facilitator, for mediating glycerol low affinity transport of the facilitated diffusion type. We present experimental evidence that this kinetics is an artefact ...
Saccharomyces cerevisiae glycerol uptake : new insights
Neves, Maria Luísa Vieira das; Lages, Fernanda; Oliveira, Rui Pedro Soares de; Brandt, Anders; Kielland-Brandt, Morten; Lucas, CândidaResumo da comunicação apresentada no "XXI International Conference on Yeast Genetics and Molecular Biology", realizada em Gotemburgo, Suécia de 7 a 12 de Julho de 2003.
Fps1p channel is the mediator of the major part of glycerol passive diffusion i...
Oliveira, Rui Pedro Soares de; Lages, Fernanda; Graça, Magda Maria Couto da Silva; Lucas, CândidaComunicação apresentada no "XXIth International Conference on Yeast Genetics and Molecular Biology", realizado em Gotemburgo, Suécia, em 2003.
Saccharomyces cerevisiae glycerol uptake : new insights
Neves, Maria Luísa Vieira das; Lages, Fernanda; Oliveira, Rui Pedro Soares de; Brandt, Anders; Kielland-Brandt, Morten; Lucas, CândidaPoster apresentado na "XXIth Yeast Genetics and Molecular Biology Conference", realizada em Gotemburgo, Suécia, de 7 a 12 de Julho de 2003.
Regulation of glycerol transport genes GUP1 and GUP2 in Saccharomyces cerevisiae
Oliveira, Rui Pedro Soares de; Lages, Fernanda; Lucas, CândidaComunicação apresentada no " 18th Small Meeting on Yeast Transport and Energetics" realizado em Ouro Preto, Brasil em 2000.
Regulation of glycerol transport genes GUP1 and GUP2 in Saccharomyces cerevisiae
Oliveira, Rui Pedro Soares de; Lages, Fernanda; Lucas, CândidaApresentação efectuada no XII Congresso Nacional de Bioquímica, na Póvoa de Varzim, em 2000.
GUP1 and its close homologue GUP2, encoding multi-membrane-spanning proteins in...
Holst, Bjørn; Lunde, Christina; Lages, Fernanda; Oliveira, Rui Pedro Soares de; Lucas, Cândida; Kielland-Brandt, MortenMany yeast species can utilise glycerol, both as sole carbon source and as an osmolyte. In Saccharomyces cerevisiae, physiological studies have previously shown the presence of an active uptake system driven by electrogenic proton symport. We have used transposon mutagenesis to isolate mutants affected in the transport of glycerol into the cell. Here we present the identification of YGL084c, encoding a multi-me...
Glycerol metabolism and transport activity regulation in Saccharomyces cerevisiae
Lages, Fernanda; Oliveira, Rui Pedro Soares de; Lucas, CândidaApresentação e resumo efectuados no 17th Small Meeting on Yeast Transport and Energetics em Córdoba, Espanha de 9 a 12 de Setembro de 1999. ; With the purpose of studying the correlation between glycerol metabolic pathway and glycerol active transport (1) in S. cerevisiae, an extensive study on glycerol transport was elaborated in all the available mutants from the genes of glycerol metabolic pathway having W3...
Isolation and characterisation of mutants from the halotolerant yeast Pichia so...
Oliveira, Rui Pedro Soares de; Lages, Fernanda; Lucas, CândidaPicha sorbitophila, a yeast species highly resistant regarding osmostress in general and to salt stress in particular, was subjected to a mutagenesis strategy in order to obtain mutants deficient in the glycerol active uptake previously described. Density centrifugation was used for enrichment of NaCl sensitive mutants in either glucose or glycerol media. Several phenotypic classes of mutants were identified, t...
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