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In vivo study of dendron-like nanoparticles for stem cells tune-up : from nano ...
Oliveira, Joaquim M.; Sousa, R. A.; Malafaya, P. B.; Silva, S. S.; Kotobuki, Noriko; Hirose, Motohiro; Ohgushi, Hajime; Mano, J. F.; Reis, R. L.The control of stem cell differentiation to obtain osteoblasts in vivo is still regarded as a challenge in stem-cell-based and bone-tissue engineering strategies. Biodegradable dexamethasone-loaded dendron-like nanoparticles (NPs) of carboxymethylchitosan/poly(amidoamine) dendrimer have been proposed as intracellular drug-delivery systems of bioactive molecules. In this study, combination of nanotechnology, ste...
Ex vivo culturing of stromal cells with dexamethasone-loaded carboxymethylchito...
Oliveira, Joaquim M.; Kotobuki, Noriko; Tadokoro, Mika; Hirose, Motohiro; Mano, J. F.; Reis, R. L.; Ohgushi, HajimeRecently, our group has proposed a combinatorial strategy in tissue engineering principles employing carboxymethylchitosan/poly(amidoamine) dendrimer nanoparticles (CMCht/PAMAM) towards the intracellular release and regimented supply of dexamethasone (Dex) aimed at controlling stem cell osteogenic differentiation in the absence of typical osteogenic inducers, in vivo. In this work, we have investigated if the D...
Macroporous hydroxyapatite scaffolds for bone tissue engineering applications :...
Oliveira, Joaquim M.; Silva, S. S.; Malafaya, P. B.; Rodrigues, Márcia T.; Kotobuki, Noriko; Hirose, Motohiro; Gomes, Manuela E.; Mano, J. F.In this work, a new methodology is reported for developing hydroxyapatite (HA) scaffolds using an organic sacrifice template. The novelty of work consists of possibility of obtaining porous and highly interconnected scaffolds mimicking the sacrificial component. Our purpose consisted of evaluating the physicochemical properties of the HA scaffolds by means of Fourier transform infra-red spectroscopy, X-ray diff...
The osteogenic differentiation of rat bone marrow stromal cells cultured with d...
Oliveira, Joaquim M.; Sousa, R. A.; Kotobuki, Noriko; Tadokoro, Mika; Hirose, Motohiro; Mano, J. F.; Reis, R. L.; Ohgushi, HajimeThere is an increasing interest in developing novel macromolecular vehicles for the intracellular and controlled delivery of bioactive molecules, since they can allow modulation of the cellular functions in a more effective manner ex vivo, and maintain the cellular phenotype in vivo upon re-implantation. The present study was designed to investigate the effect of combining novel dexamethasone-loaded carboxymeth...
Surface engineered carboxymethylchitosan/poly(amidoamine) dendrimer nanoparticl...
Oliveira, Joaquim M.; Kotobuki, Noriko; Marques, A. P.; Pirraco, Rogério; Benesch, Johan; Hirose, Motohiro; Costa, Silgia; Mano, J. F.; Hajime, OhgushiNovel highly branched biodegradable macromolecular systems have been developed by grafting carboxymethylchitosan (CMCht) onto low generation poly(amidoamine) (PAMAM) dendrimers. Such structures organize into sphere-like nanoparticles that are proposed to be used as carriers to deliver bioactive molecules aimed at controlling the behavior of stem cells, namely their proliferation and differentiation. The nanopar...
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