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Molecular approach to characterize ectomycorrhizae fungi from Mediterranean pin...
Ragonezi, Carla; Caldeira, A. Teresa; Martins, M. Rosário; Salvador, Cátia; Santos-Silva, Celeste; Ganhão, Elsa; Klimaszewska, KrystynaStone pine (Pinus pinea L.), like other conifers, forms ectomycorrhizas (ECM), which have beneficial impact on plant growth in natural environments and forest ecosystems. An in vitro co-culture of stone pine microshoots with pure mycelia of isolated ECM sporocarps was used to overcome the root growth cessation not only in vitro but also to improve root development during acclimation phase. Pisolithus arhizus (S...
O-coumaric acid ester, a potential early signaling molecule in Pinus pinea and ...
Ragonezi, Carla; Teixeira, Dora; Caldeira, Ana Teresa; Martins, Maria do Rosário; Santos Silva, Celeste; Ganhão, Elsa; Klimaszewska, KrystynaDuring ectomycorrhizal (ECM) establishment, biochemical signals lead to the development of complex structures in both the plant and the fungus that ultimately result in the formation of an ectomycorrhiza. The cross-talk between partners begins before physical contact. Our objective was to investigate the chemical nature of the signals during the first stages of in vitro mycorrhization of Pinus pinea with Pisoli...
Micropropagation of recalcitrant pine (Pinus pinea L) an overview of the effect...
Ragonezi, Carla; Caldeira, Ana Teresa; Martins, María do Rosário; Teixeira, Dora; Silva Dias, Luís; Miralto, Maria Otília; Ganhão, ElsaOral presentation Micropropagation of recalcitrant pine (Pinus pinea L.). An overview of the effects of ectomycorrhizal inoculation Carla Ragonezi1; Ana Teresa Caldeira2 3; Maria do Rosário Martins2,5; Otília Miralto5,6; Luís Silva Dias6; Elsa Ganhão6, Krystyna Klimaszewska4, Amely Zavattieri5,6 1 Plant Breeding and Biotechnology Laboratory, University of Évora, Ap. 94, 7002-554 Évora, Portugal 2 Department o...
Adventitious rooting of conifers: influence of physical and chemical factors
Ragonezi, Carla; Klimaszewska, Krystyna; Castro, Mário Rui; Lima, Mónica; Oliveira, Paulo; Zavattieri, Maria AmelyIn conifers, vegetative propagation of superior genotypes is the most direct means for making large genetic gains, because it allows a large proportion of genetic diversity to be captured in a single cycle of selection. There are two aims of vegetative propagation, namely large-scale multiplication of select genotypes and production of large numbers of plants from scarce and costly seed that originates from con...
Influence of light quality and intensity on adventitious root formation in micr...
Ragonezi, Carla; Castro, Mário Rui Basílio; Klimaszewska, Krystyna; Lima, Mónica; Zavattieri, Maria AmelyIn the present study of Pinus pinea L., further improvement of microshoot rooting was achieved by applying Cool-white light at increased intensity from 60 to 90 µmol m-2 s-1. In contrast, light provided by Gro-lux lamps promoted rooting of the microshoots at the same frequency regardless of its intensity. Majority of microshoots (70.4%) grown under Cool-white lamps at the intensity of 90 µmol m-2 s-1 were also ...
Mycorrhiza-like structures in rooted microshoots of Pinus pinea L.
Castro, Mário Rui da Costa Basílio e; Ragonezi Gomes Lopes, Carla Aparecida; Klimaszewska, Krystyna; Lima, Mónica; Oliveira, Paulo Guilherme dePinus pinea L. (stone pine) is one of the major plantation species in Iberian Peninsula, being Portugal the largest edible seed producer in the world. The induction and improvement of in vitro rhizogenesis of microshoots of Pinus pinea was developed in our laboratory using a co-culture system with ECM fungi. In the acclimation phase in mixed substrates, or in rhizotrons, anatomical and morphological studies wer...
Adventitious rooting of conifers: influence of physical and chemical factors
Ragonezi, Carla; Klimaszewska, Krystyna; Castro, Mário Rui da Costa Basílio e; Lima, Mónica; Oliveira, Paulo Guilherme de; Zavattieri, Maria AmelyIn conifers, vegetative propagation of superior genotypes is the most direct means for making large genetic gains, because it allows a large proportion of genetic diversity to be captured in a single cycle of selection. There are two aims of vegetative propagation, namely large-scale multiplication of select genotypes and production of large numbers of plants from scarce and costly seed that originates from con...
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