This work presents a multi-route, nonstructural kinetic model for characterization of ethanol fermentation of lactose using a recombinant flocculent Saccharomyces cerevisiae strain expressing both the LAC4 (coding for β-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces lactis. In this model, the values of different metabolic pathways are calculated applying a modified Monod equation...
This work presents a multi-route, non-structural kinetic model for interpretation of ethanol fermentation of lactose using a recombinant flocculent Saccharomyces cerevisiae strain expressing both the LAC4 (coding for β-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces lactis. In this model, the values of different metabolic pathways are calculated applying a modified Monod equation r...
A low-cost and simple magnetic particle tracer method was adapted to characterize the hydrodynamic behavior of an internal- and an external-loop airlift reactor (ALR). The residence time distribution of three magnetic particles differing in diameter (5.5, 11.0 and 21.2mm) and with a density very close to that of water was measured in individual reactor sections. The measured data were analyzed and used to deter...
In recent years, there has been a growing interest in bioreactors utilizing immobilized or flocculating cells in continuous process in order to improve the bioprocess productivity. One of possible promising implementations of continuous flocculation yeast system is bioremediation of cheese whey by means of alcoholic fermentation of lactose. The aim of this work was to carry out a kinetic analysis of alcoholic f...
In this article a hydrodynamic and rheological analysis of a continuous airlift bioreactor with high-celldensity system is presented. A highly flocculating recombinant strain of Sacharomyces cerevisiae containing genes for lactose transport (lactose permease) and hydrolysis (β-galactosidase) was exploited to ferment lactose from cheese whey to ethanol. The magnetic particle-tracer method was used to assess the ...
One of the most common ways to improve the productivity of a fermentation process is the use of high cell density systems. In practice, such a system usually represents a threephase (gas-liquid-solid) dispersion operating in a continuous mode. The interest for these biosystems has been increasing because they seem to be a very promising alternative to the traditional batch fermentation with freely suspended cel...
In this paper a hydrodynamic and rheological analysis of a continuous airlift bioreactor with high cell density system is presented. A highly flocculating recombinant strain of Sacharomyces cerevisae containing genes for lactose transport (lactose permease) and hydrolysis (β-galactosidase) was exploited to ferment lactose from cheese whey to ethanol. The magnetic particle-tracer method was used to assess the ef...
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