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Gradual pore formation in natural origin scaffolds throughout subcutaneous impl...
Martins, Ana M.; Kretlow, J. D.; Pinto, A. R.; Malafaya, P. B.; Fernandes, E. M.; Neves, N. M.; Alves, Catarina M.; Mikos, Antonios G.This study used a rat subcutaneous implantation model to investigate gradual in situ pore formation in a self-regulating degradable chitosan-based material, which comprises lysozyme incorporated into biomimetic calcium phosphate (CaP) coatings at the surface to control the scaffold degradation and subsequent pore formation. Specifically, the in vivo degradation of the scaffolds, the in situ pore formation, and ...
Responsive and In situ-forming chitosan scaffolds for bone tissue engineering a...
Martins, Ana M.; Alves, C. M.; Kasper, F. Kurtis; Mikos, Antonios G.; Reis, R. L.The use of bioabsorbable polymeric scaffolds is being investigated for use in bone tissue engineering applications, as their properties can be tailored to allow them to degrade and integrate at optimal rates as bone remodelling is completed. The main goal of this review is to highlight the “intelligent” properties exhibited by chitosan scaffolds and their use in the bone tissue engineering field. To complement ...
Combination of enzymes and flow perfusion conditions improves osteogenic differ...
Martins, Ana M.; Saraf, A.; Sousa, R. A.; Alves, C. M.; Mikos, Antonios G.; Kasper, F. Kurtis; Reis, R. L.Previous studies have shown that a-amylase and lipase are capable of enhancing the degradation of fiber meshes blends of starch and poly(e-caprolactone) (SPCL) under dynamic conditions, and consequently to promote the proliferation and osteogenic differentiation of bone marrow stromal cells (MSCs). This study investigated the effect of flow perfusion bioreactor culture in combination with enzymes on the osteoge...
The role of lipase and alpha-amylase in the degradation of starch/poly(epsilon-...
Martins, Ana M.; Pham, Q. P.; Malafaya, P. B.; Sousa, R. A.; Gomes, Manuela E.; Raphael, R. M.; Kasper, F. Kurtis; Reis, R. L.; Mikos, Antonios G.The present work studies the influence of hydrolytic enzymes (a-amylase or lipase) on the degradation of fiber mesh scaffolds based on a blend of starch and poly(e-caprolactone) (SPCL) and the osteogenic differentiation of osteogenic medium–expanded rat bone marrow stromal cells (MSCs) and subsequent formation of extracellular matrix on these scaffolds under static culture conditions. The biodegradation profile...
Natural stimulus responsive scaffolds/cells for bone tissue engineering : influ...
Martins, Ana M.; Pham, Q. P.; Malafaya, P. B.; Raphael, R. M.; Kasper, F. Kurtis; Reis, R. L.; Mikos, Antonios G.This work proposes the use of nonporous, smart, and stimulus responsive chitosan-based scaffolds for bone tissue engineering applications. The overall vision is to use biodegradable scaffolds based on chitosan and starch that present properties that will be regulated by bone regeneration, with the capability of gradual in situ pore formation. Biomimetic calcium phosphate (CaP) coatings were used as a strategy t...
Toward osteogenic differentiation of marrow stromal cells and in vitro producti...
Martins, Ana M.; Alves, C. M.; Reis, R. L.; Mikos, Antonios G.; Kasper, F. KurtisUncorrected proof ; Tissue engineering has emerged as a new interdisciplinary field for the repair of various tissues, restoring their functions by using scaffolds, cells, and/or bioactive factors. A temporary scaffold acts as an extracellular matrix analog to culture cells and guide the development of new tissue. In this chapter, we discuss the preparation of naturally derived scaffolds of polysaccharide orig...
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