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A novel metalloprotease from Bacillus cereus for protein fibre processing
Sousa, Fernanda; Jus, S.; Erbel, Anita; Kokol, V.; Paulo, Artur Cavaco; Gübitz, Georg M.A novel protease produced by Bacillus cereus grown on wool as carbon and nitrogen source was purified. B. cereus protease is a neutral metalloprotease with a molecular mass of 45.6 kDa. The optimum activity was at 45 °C and pH 7.0. The substrate specificity was assessed using oxidized insulin B-chain and synthetic peptide substrates. The cleavage of the insulin B-chain was determined to be Asn3, Leu6, His10-Leu...
A new cuticle scale hydrolysing protease from beauveria brongniartii
Erlacher, Angelika; Sousa, Fernanda; Schroeder, M.; Jus, S.; Kokol, V.; Paulo, Artur Cavaco; Gübitz, Georg M.From a screening for the production of new proteases specific for cuticle scales, Beauveria brongniartii was selected producing an alkaline Ca++ dependent protease. The purified had a molecular weight of 27 kDa and a pI value of 8.0. Substrate specificities of model substrates (wool with partially removed cuticles treated with SDS) were analyzed by protein release, dissolved organic carbon (DOC) and nitrogen an...
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