Encontrados 9 documentos, a visualizar página 1 de 1
Rapeseed oil‑rich diet alters hepatic mitochondrial membrane lipid composition ...
Monteiro, João P.; Pereira, Cláudia V.; Silva, Ana M.; Maciel, Elisabete; Baldeiras, Inês; Peixoto, Francisco; Domingues, Maria R.; Jurado, Amália S.Diet is directly related with physiological alterations occurring at a cell and subcellular level. However, the role of diet manipulation on mitochondrial physiology is still largely unexplored. Aiming at correlating diet with alterations of mitochondrial membrane composition and bioenergetics, Wistar-Han male rats were fed for 11, 22 and 33 days with a rapeseed oil-based diet and mitochondrial bioenergetics, a...
S4(13)-PV cell-penetrating peptide induces physical and morphological changes i...
Cardoso, Ana M. S.; Trabulo, Sara; Cardoso, Ana L.; Lorents, Annely; Morais, Catarina M.; Gomes, Paula; Nunes, Cláudia; Lúcio, Marlene; Reis, SaletteThe present work aims to gain insights into the role of peptide–lipid interactions in the mechanisms of cellular internalization and endosomal escape of the S4(13)-PV cell-penetrating peptide, which has been successfully used in our laboratory as a nucleic acid delivery system. A S4(13)-PV analogue, S4(13)-PVscr, displaying a scrambled amino acid sequence, deficient cell internalization and drug delivery inabil...
Controlling the Morphology in DNA Condensation and Precipitation
Pinto, Maria F. V.; Morán, M. Carmen; Miguel, M. Graça; Lindman, Björn; Jurado, Amália S.; Pais, Alberto A. C. C.This work addresses the influence of solution inhomogeneity on conformation, aggregation, and coil/globule and bundle/single chain coexistence of T4 DNA molecules. The inhomogeneity is induced by mixing two solutions containing, respectively, protamine and DNA, with different relative concentrations, but aiming at producing the same final concentrations. The study was conducted by means of fluorescence microsco...
Comparative effects of three 1,4-dihydropyridine derivatives [OSI-1210, OSI-121...
Fernandes, Maria A. S.; Pereira, Susana P. S.; Jurado, Amália S.; Custódio, José B. A.; Santos, Maria S.; Moreno, António J. M.; Duburs, GunarsThe 1,4-dihydropyridines OSI-1210, OSI-1211 (etaftoron), and OSI-3802 are compounds with similar chemical structure. They differ by the length of the alkoxyl chain in positions 3 and 5 of the dihydropyridine (DHP) ring and by their pharmacological action characteristics. However, as far as we know, a clear relationship between the effects of these compounds and the length of the alkoxyl chain in positions 3 and...
Non-Selective Toxicological Effects of the Insect Juvenile Hormone Analogue Met...
Monteiro, João; Videira, Romeu; Matos, Manuel; Dinis, Augusto; Jurado, AmáliaAbstract The Gram-positive bacterium, Bacillus stearothermophilus, was used as a model organism to identify the non-selective toxic effects of the currently used insecticide methoprene (isopropyl(2E,4E)-11-methoxy-3,7,11-trimethyl-2,4-dodecadienoate). A significant decrease of the yield of bacterial cultures and a premature appearance of ultrastructural abnormalities in cells cultured in the presence of the in...
Lipid composition and dynamics of cell membranes of Bacillus stearothermophilus...
Rosa, Sónia M. L. J.; Antunes-Madeira, Maria do Carmo; Matos, Manuel J.; Jurado, Amália S.; Madeira, Vítor M. C.Bacillus stearothermophilus, a useful model to evaluate membrane interactions of lipophilic drugs, adapts to the presence of amiodarone in the growth medium. Drug concentrations in the range of 1-2 [mu]M depress growth and 3 [mu]M completely suppresses growth. Adaptation to the presence of amiodarone is reflected in lipid composition changes either in the phospholipid classes or in the acyl chain moieties. Sign...
Amiodarone interactions with membrane lipids and with growth of Bacillus stear...
Rosa, Sónia; Antunes-Madeira, Maria; Jurado, Amália; Madeira, VítorAbstract The thermophilic eubacterium Bacillus stearothermophilus was used as a model to study the effects of amiodarone (2-butyl-3-[3',5'diido-4'a-diethyl-aminoethoxybenzoyl]-benzofuran) in lipid organization and in bacterial growth. Effects on the structural order of lipids were assessed by fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH), probing the bilayer core, and of the propionic acid d...
Toxicity assessment of tamoxifen by means of a bacterial model
Luxo, Cristina; Jurado, Amália; Madeira, VítorAbstract A strain of Bacillus stearothermophilus was used as a model to study physical perturbations induced in the membrane by the cytostatic tamoxifen (TAM). This study was carried out using two lines of criteria: (1) bacterial growth, and temperature growth range, with determination of growth parameters as a function of TAM concentration; and (2) biophysical studies by differential scanning calorimetry (DSC...
Lipid composition changes induced by tamoxifen in a bacterial model system
Luxo, Cristina; Jurado, Amália S.; Madeira, Vítor M. C.A putative relationship between growth impairment of Bacillus stearothermophilus by tamoxifen (TAM) and TAM-induced perturbation of the physical properties of bacterial membrane lipids has been observed. The supplementation of the growth medium with Ca2+ (a membrane stabilizer) partially relieves growth inhibition by TAM, allowing growth at TAM concentrations that fully impair growth in the basal medium. B. ste...
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