Encontrados 11 documentos, a visualizar página 1 de 2
Application of Thermoresponsive PNIPAAM‑b‑PAMPTMA Diblock Copolymers in siRNA D...
Cardoso, Ana M.; Calejo, M. Teresa; Morais, Catarina M.; Cardoso, Ana L.; Cruz, Rita; Zhu, Kaizheng; de Lima, M. C. P.; Jurado, A. S.; Nyström, BoGene knockdown has emerged as an important tool for cancer gene therapy as well as for viral infections and dominantly inherited genetic disorders. The generation of suitable siRNA delivery systems poses some challenges, namely, to avoid nuclease degradation, to surpass the cytoplasmic membrane, and to release the nucleic acids into the cytosol. Aiming at evaluating the ability of thermoresponsive block copolym...
Sustained Release of Naltrexone from Poly(N-Isopropylacrylamide) Microgels
Kjoniksen, A.L.; Calejo, M.T.; Zhu, K.Z.; Cardoso, A.M.S.; de Lima, M.C.P.; Jurado, A.S.; Nystrom, B.; Sande, S.A.The release of the opioid antagonist naltrexone from neutral poly(N-isopropylacrylamide) (PNIPAAM)microgels and negatively charged PNIPAAM microgels containing acrylic acid groups (PNIPAAM-co-PAA) has been studied at various microgel and drug concentrations. The release curves were found to be well represented by the Weibull equation. The release rates were observed to be dependent on the microgel concentration...
Mitochondrial membrane lipid remodeling in pathophysiology: A new target for di...
Monteiro, João P.; Oliveira, Paulo J.; Jurado, A. S.Mitochondria are arbiters in the fragile balance between cell life and death. These organelles present an intricate membrane system, with a peculiar lipid composition and displaying transverse as well as lateral asymmetry. Some lipids are synthesized inside mitochondria, while others have to be imported or acquired in the form of precursors. Here, we review different processes, including external interventions ...
Studies on the toxicity of an aqueous suspension of C60 nanoparticles using a b...
Santos, Sandra M. A.; Dinis, A. M.; Rodrigues, David M. F.; Peixoto, Francisco; Videira, Romeu A.; Jurado, A.S.The increasing use of C60 nanoparticles and the diversity of their applications in industry and medicine has led to their production in a large scale. C60 release into wastewaters and the possible accumulation in the environment has raised concerns about their ecotoxicological impact. In the present study, an aqueous suspension of C60 nanoparticles was prepared and its potential toxicity studied in laboratory, ...
Comparative study of tributyltin toxicity on two bacteria of the genus Bacillus
Martins, J. D.; Jurado, A. S.; Moreno, A. J. M.; Madeira, V. M. C.Tributyltin is a potent biocide mainly used in marine antifouling paints. Owing to its widespread distribution in coast areas and its high toxicity to aquatic organisms, the use of this compound is generally restricted and under government regulation. Despite of that, it persists in the aquatic environment. Organotins used in industry have also been detected in terrestrial environments. The persistence and high...
Toxicity of methoprene as assessed by the use of a model microorganism
Monteiro, J. P.; Jurado, A. S.; Moreno, A. J. M.; Madeira, V. M. C.Methoprene is an insect juvenile growth hormone mimic, commonly used as a pesticide. Although widely used for the control of several pests, toxic effects on organisms of different phyla have been reported. These events triggered studies to clarify the mechanisms of toxicity of this insecticide putatively involved in ecological issues. Here we show the effect of methoprene on the normal cell growth and viability...
Use of the microorganism Bacillus stearothermophilus as a model to evaluate tox...
Martins, J. D.; Monteiro, J. P.; Antunes-Madeira, M. C.; Jurado, A. S.; Madeira, V. M. C.Microorganisms are very powerful tools for the supply of information about the toxic effects of lipophilic compounds, since an impairment of cell growth usually occurs as a result of perturbations related, in most cases, with the partition of toxicants in membranes. The thermophilic eubacterium Bacillus stearothermophilus has been used as a model system to identify [alpha]- and [beta]-endosulfan interactions wi...
Tamoxifen induces ultrastructural alterations in membranes of Bacillus Stearoth...
Luxo, C.; Jurado, A. S.; Madeira, Vítor M. C.; Silva, M. T.Tamoxifen (TAM), a non-steroid antiestrogen, is the mostly used drug for chemotherapy and chemoprevention of breast cancer. However, the mechanisms by which TAM inhibits cell proliferation in breast cancer are not fully understood. TAM strongly incorporates in biomembranes and a variety of effects have been assigned to biophysical and biochemical interactions with membranes. Therefore, a better understanding of...
Molecular mechanisms of the metabolite 4-hydroxytamoxifen of the anticancer dru...
Monteiro, J. P.; Martins, J. D.; Luxo, P. C.; Jurado, A. S.; Madeira, V. M. C.A strain of the thermophilic eubacterium Bacillus stearothermophilus was used as a model system to identify membrane mediated cytotoxic effects of 4-hydroxytamoxifen, following previous studies with tamoxifen. With this experimental approach we attempted to further clarify tamoxifen and 4-hydroxytamoxifen membrane interactions often evoked as responsible for their multiple cellular effects. Bacterial growth and...
Toxic effects of tamoxifen on the growth and respiratory activity of Bacillus s...
Luxo, C.; Jurado, A. S.; Custódio, J. B. A.; Madeira, V. M. C.The anticancer drug tamoxifen (TAM) is used as first line therapy in breast cancer. Although tamoxifen is usually considered an estrogen antagonist, several studies suggest alternative mechanisms of action. Bacillus stearothermophilus has been used as a model to clarify the antiproliferative action of tamoxifen putatively related with drug-membrane interaction. According to previous data, TAM induces perturbati...
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