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Proteomic approach to Pseudomonas aeruginosa adaptive resistance to benzalkoniu...
Machado, Idalina; Coquet, Laurent; Jouenne, Thierry; Pereira, M. O.This study aimed to assess the membrane modifications in Pseudomonas aeruginosa after continuous exposure to increasing doses of benzalkonium chloride (BC). Two different concentrations were used, 0.9 and 12.0 mM. Proteomic investigations revealed that the range of the outermembrane proteome alterations following continuous exposure is very low, i.e. about 10% and BC concentration dependent. Adapted cells revea...
Proteomic characterization of Benzalkonium Chloride- and Ciprofloxacin-adapted ...
Machado, Idalina; Coquet, Laurent; Jouenne, Thierry; Pereira, M. O.Bacteria are able to adapt to several environmental stresses such as the presence of antimicrobial molecules and, as consequence, bacterial resistance may increase with increasing exposure to antimicrobials. The most impressive mechanism of the bacterial mode of life is their grow as part of a sessile community referred to as biofilm [1]. Biofilm formation is an important aspect of many bacterial diseases, espe...
OMP proteomic analysis of benzalkonium chloride and ciprofloxacin adapted biofi...
Machado, Idalina; Pereira, M. O.; Jouenne, ThierryAdaptive resistance to antimicrobials has been widely reported in planktonic studied trough phenotypic characterization and proteomic analysis. Concerning biofilm adaptation, the response of biofilm-entrapped cells to chemical stress conditions is not yet well studied. There is evidence that proteins involved in oxidative stress response, cell envelope synthesis, as well as in synthesis of EPS become up- or dow...
Membranome analysis of benzalkonium chloride adapted planktonic and biofilm cells
Machado, Idalina; Jouenne, Thierry; Pereira, M. O.Este resumo faz parte de: Book of abstracts of the Meeting of the Institute for Biotechnology and Bioengineering, 2, Braga, Portugal, 2010. A versão completa do livro de atas está disponível em: http://hdl.handle.net/1822/10968
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