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Sources of hepatic glucose production by 2H2O ingestion and Bayesian analysis o...

Delgado, TC; Barosa, C; Castro, MM; Geraldes, CF; Bastos, M; Baptista, C; Fagulha, A; Barros, L; Mota, A; Carvalheiro, M; Jones, JG; Merrit, M

The contribution of gluconeogenesis to hepatic glucose production (GP) was quantified after (2)H(2)O ingestion by Bayesian analysis of the position 2 and 5 (2)H-NMR signals (H2 and H5) of monoacetone glucose (MAG) derived from urinary acetaminophen glucuronide. Six controls and 10 kidney transplant (KTx) patients with cyclosporine A (CsA) immunosuppressant therapy were studied. Seven KTx patients were lean and ...


Noninvasive analysis of hepatic glycogen kinetics before and after breakfast wi...

Jones, JG; Fagulha, A; Barosa, C; Bastos, M; Barros, L; Baptista, C; Caldeira, MM; Carvalheiro, M

The contributions of hepatic glycogenolysis to fasting glucose production and direct pathway to hepatic glycogen synthesis were quantified in eight type 1 diabetic patients and nine healthy control subjects by ingestion of (2)H(2)O and acetaminophen before breakfast followed by analysis of urinary water and acetaminophen glucuronide. After overnight fasting, enrichment of glucuronide position 5 relative to body...


Sources of glucose production in cirrhosis by 2H2O ingestion and 2H NMR analysi...

Perdigoto, R; Furtado, AL; Porto, A; Rodrigues, TB; Geraldes, CF; Jones, JG

Plasma glucose 2H enrichment was quantified by 2H NMR in patients with cirrhosis (n=6) and healthy subjects (n=5) fasted for 16 h and given 2H(2)O to approximately 0.5% body water. The percent contribution of glycogenolysis and gluconeogenesis to glucose production (GP) was estimated from the relative enrichments of hydrogen 5 and hydrogen 2 of plasma glucose. Fasting plasma glucose levels were normal in both g...


Integration of [U-13C]glucose and 2H2O for quantification of hepatic glucose pr...

Perdigoto, R; Rodrigues, TB; Furtado, AL; Porto, A; Geraldes, CF; Jones, JG

Glucose metabolism in five healthy subjects fasted for 16 h was measured with a combination of [U-13C]glucose and 2H2O tracers. Phenylbutyric acid was also provided to sample hepatic glutamine for the presence of 13C-isotopomers derived from the incorporation of [U-13C]glucose products into the hepatic Krebs cycle. Glucose production (GP) was quantified by 13C NMR analysis of the monoacetone derivative of plasm...


Integration of [U-13C]glucose and 2H2O for quantification of hepatic glucose pr...

Perdigoto, R; Rodrigues, TB; Furtado, AL; Porto, A; Geraldes, CF; Jones, JG

Glucose metabolism in five healthy subjects fasted for 16 h was measured with a combination of [U-13C]glucose and 2H2O tracers. Phenylbutyric acid was also provided to sample hepatic glutamine for the presence of 13C-isotopomers derived from the incorporation of [U-13C]glucose products into the hepatic Krebs cycle. Glucose production (GP) was quantified by 13C NMR analysis of the monoacetone derivative of plasm...


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