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Analysis of DNA damage and repair in Saccharomyces cerevisiae using the comet a...

In this work we used the model organism Saccharomyces cerevisiae to characterise the biological activity and the mechanism of action of phytochemicals. We have assessed DNA damage and repair using the comet assay, which displayed a dose-response relationship with different DNA-damaging agents. Subsequently, we used this system to assess the antigenotoxic properties of a leaf extract from Ginkgo biloba (GBE). Ex...

Suppressing fatty acid uptake by a fatty acid secreting saccharomyces cerevisia...

Biodiesel, in the form of fatty acid esters, produced by oleaginous microorganisms, could represent an attractive alternative of renewable liquid biofuels. Lipid metabolism has been studied extensively in S. cerevisiae and all genes encoding enzymes directly involved in lipid synthesis are known. In this model yeast, exogenous long-chain fatty acids are activated to coenzyme A derivatives prior to metabolic uti...

Delayed fatty acid uptake by a fatty acid secreting yeast strain with modified ...

Biodiesel, in the form of fatty acid esters, produced by oleaginous microorganisms, could represent an attractive alternative of renewable liquid biofuels. In the model yeast S. Cerevisiae lipid metabolism has been studied extensively and all genes encoding enzymes directly involved in lipid synthesis are known. In this organism exogenous long-chain fatty acids are activated to coenzyme A derivatives prior to m...

Screening for interactions with predicted soluble domains of the yeast lactate ...

The S. cerevisiae membrane protein Jen1 is a monocarboxylate-proton symporter which after the addition of glucose to lactic acid-grown cells triggers loss of Jen1p activity and repression of JEN1 gene expression (Paiva et al., 2002; Andrade et al., 2001). This downregulation of the permease is dependent on phosphorylation and ubiquitylation mechanisms, both of which are dependent on proteins interacting with th...

A functional screening assay for aldopentose transport in Saccharomyces cerevisiae

D-Xylose and L-arabinose are two important aldopentose sugars present in lignocellulosic hydrolysates, which is a major feedstock for 2nd generation bioethanol production. Saccharomyces cerevisiae is the proferred ethanol production organism but lacks efficient pentose transport and metabolism. Heterologous xylose and arabinose metabolic genes have been successfully expressed in S. cerevisiae, enabling growth o...

Measuring oxidative DNA damage and DNA repair using the yeast comet assay

Chromosomal DNA damage can be a result of several processes and agents of endogenous or exogenous origin. These cause strand breaks or oxidized bases that lead to strand breaks, which relax the normally supercoiled genomic DNA and increase its electrophoretic mobility. The extent of DNA damage can be assessed by single cell gel electrophoresis, where the chromosomal DNA migration distance correlates with the ex...

Analysis of DNA damage and repair in saccharomyces cerevisiae using the comet a...

In this work we used the model organism Saccharomyces cerevisiae to characterise the biological activity and the mechanism of action of phytochemicals. One of the goals is to use mutant strains affected in basic mechanisms of oxidative stress response and DNA repair in order to uncover the molecular targets of phytochemicals. We have assessed DNA damage and repair using the comet assay, evaluated as “comet tail...

Evaluation of prevention of DNA damage and induction of DNA repair in Saccharom...

Extracts of Ginkgo biloba leaves (GBE) have been used for centuries in traditional oriental medicine to treat a very wide range of ailments. These extracts contain flavone glycosides, terpene trilactones (ginkgolides and bilobalide), ginkgolic acids, proanthocyanides and other uncharacterized compounds. The flavone glycosides and terpene trilactones fractions are believed to be responsible for the pharmacologic...

MAPK signaling pathways mediate acetic acid-induced cell death in saccharomyces...

Plasmids for in vivo construction of integrative Candida albicans vectors in sa...

A general system has been devised for the in vivo construction of Candida albicans integrative vectors in Saccharomyces cerevisiae. The system is especially useful for the integration of genes in C. albicans that cannot be propagated in Escherichia coli possibly because of their toxic effects. The ligation of S. cerevisiae 2μ sequences to a C. albicans integrative vector permits in vivo maintenance and gap repa...