To evaluate the antifungal effect of gallic acid, catechin, luteolin and quercetin, phenolic compounds identified from flowers of North Eastern Portugal, against Candida planktonic and biofilm cells. Materials & methods: The MICs were determined in Candida planktonic cells and the effect of phenolic compounds on Candida biofilms was assessed through quantification of CFUs. Results: MIC values demonstrated that ...
Currently, mammalian cell technology has become the focus of biopharmaceutical production, with strict regulatory scrutiny of the techniques employed. Major concerns about the presence of animal-derived components in the culture media led to the development of serum-free (SF) culture processes. However, cell adaptation to SF conditions is still a major challenge and limiting step of process development. Thus, t...
Candida tropicalis has been reported to be one of the Candida species which is most likely to cause bloodstream and urinary tract infections in hospitals. Several virulence factors seem to be responsible for C. tropicalis infections, which present high potential for dissemination and mortality. The aim of this study was to investigate the correlation between different virulence factors (enzymes secretion, adhes...
Este artigo apresenta e discute alguns resultados sobre a evolução dos procedimentos de cálculo usados por alunos de uma turma do 3.º ano de escolaridade, na resolução de tarefas de multiplicação. Estes resultados integram um estudo mais abrangente que teve como propósito a compreensão do modo como os alunos aprofundam a aprendizagem da multiplicação numa perspetiva de desenvolvimento do sentido de número, no â...
As creches assumem, atualmente, um importante papel na sociedade no que diz respeito ao bem-estar e ao bom desenvolvimento das crianças dos 0 aos 3 anos. O presente trabalho aborda as funções assumidas pelo diretor técnico enquanto líder de uma equipa de trabalho e analisa a perceção que delas têm dirigentes e funcionários de um conjunto de creches. Teve-se em conta uma primeira análise teórica dos diferentes t...
Disruption of E-cadherin (CDH1 gene) expression, subcellular localization or function arises during initiation and progression of almost 90% of all epithelial carcinomas. Nevertheless, the mechanisms through which this occurs are largely unknown. Previous studies showed the importance of CDH1 intron 2 sequences for proper gene and protein expression, supporting these as E-cadherin cis-modulators. Through RACE a...
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