Encontrados 32 documentos, a visualizar página 1 de 4
Temporal changes in macrofauna as response indicator to potential human pressur...
Bessa, Filipa; Gonçalves, Sílvia C.; Franco, João N.; André, José N.; Cunha, Pedro P.; Marques, J. C.Sandy beaches are natural dynamic ecosystems, which are becoming worldwide increasingly disturbed by intensive human direct use, coastal development and erosive evolution. In this study, we have examined whether ten years of potential increased human pressures have resulted in significant changes in the macrofaunal assemblages’ structure and composition of two mesotidal sandy beaches (Cabedelo – urban beach, an...
Tumor-targeted Chlorotoxin-coupled Nanoparticles for Nucleic Acid Delivery to G...
Costa, Pedro M.; Cardoso, Ana L.; Mendonça, Liliana S.; Serani, Angelo; Custódia, Carlos; Conceição, Mariana; Simões, Sérgio; Moreira, João N.The present work aimed at the development and application of a lipid-based nanocarrier for targeted delivery of nucleic acids to glioblastoma (GBM). For this purpose, chlorotoxin (CTX), a peptide reported to bind selectively to glioma cells while showing no affinity for non-neoplastic cells, was covalently coupled to liposomes encapsulating antisense oligonucleotides (asOs) or small interfering RNAs (siRNAs). T...
Impact of anti-PLK1 siRNA-containing F3-targeted liposomes on the viability of ...
Gomes-da-Silva, Lígia C.; Ramalho, José S.; de Lima, M. C. P.; Simões, Sérgio; Moreira, João N.We have previously described the development of novel sterically stabilized F3-targeted pH-sensitive liposomes, which exhibited the ability to target both cancer and endothelial cells. Herein, the therapeutic potential of those liposomes was assessed upon encapsulation of a siRNA against a well-validated molecular target, PLK1. Treatment of prostate cancer (PC3) and angiogenic endothelial (HMEC-1) cells with F3...
On the use of dexamethasone-loaded liposomes to induce the osteogenic different...
Monteiro, Nelson; Martins, Albino; Ribeiro, Diana Margarida da Costa; Faria, Susana, 1972-; Fonseca, Nuno A.; Moreira, João N.; Reis, R. L.Stem cells have received considerable attention by the scientific community because of their potential for tissue engineering and regenerative medicine. The most frequently used method to promote their differentiation is supplementation of the in vitro culture medium with growth/differentiation factors (GDFs). The limitations of that strategy caused by the short half-life of GDFs limit its efficacy in vivo and ...
Immobilization of bioactive factor-loaded liposomes at the surface of electrosp...
Monteiro, Nelson; Martins, Albino; Pires, R. A.; Faria, Susana, 1972-; Fonseca, Nuno A.; Moreira, João N.; Reis, R. L.; Neves, N. M.Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013) ; The ability to manipulate and control the surface properties is of crucial importance in the designing of scaffolds for Tissue Engineering (TE) and Regenerative Medicine. Electrospun nanofibers (NFM), due to their morphology and fibrous structure have received much attention as potential biomedical devices, TE scaf...
VScore: uma plataforma para desenvolvimento de novos fármacos usando screening ...
Froufe, Hugo J.C.; Ferreira, Isabel C.F.R.; Sousa, João N.; Amaro, José C.R.; Abreu, Rui M.V.O screening virtual de grandes bibliotecas de compostos químicos, utilizando metodologias computacionais como o molecular docking, é cada vez mais utilizado na procura de potenciais inibidores de proteínas-alvo envolvidas em diferentes patologias. No entanto, devido ao facto destas bibliotecas poderem atingir um número de compostos químicos que podem ir das dezenas às centenas de milhar, é muitas vezes necessár...
Towards a siRNA-containing nanoparticle targeted to breast cancer cells and the...
Gomes-da-Silva, Lígia C.; Santos, Adriana O.; Bimbo, Luís M.; Moura, Vera; Ramalho, José S.; Lima, Maria C. Pedroso de; Simões, Sérgio; Moreira, João N.The present work aimed at designing a lipid-based nanocarrier for siRNA delivery towards two cell sub-populations within breast tumors, the cancer and the endothelial cells from angiogenic tumor blood vessels. To achieve such goal, the F3 peptide, which is specifically internalized by nucleolin overexpressed on both those sub-populations, was used as a targeting moiety. The developed F3-targeted stable nuc...
Induction of human mesenchymal stem cells osteogenesis by bioactive agent-relea...
Monteiro, Nelson; Martins, Albino; Ribeiro, Diana Margarida da Costa; Faria, Susana, 1972-; Fonseca, Nuno A.; Moreira, João N.; Reis, R. L.Stem cell therapy is a rapidly evolving area of research in regenerative medicine. Mesenchymal stem cells (MSCs) have received considerable attention by the scientific community because of their potential of expansion and the ability to differentiate into various mesodermal tissues. Liposomes are well-established non-viral carrier systems, presenting significant advantages over other nanoparticle-based drug del...
Microsatellite instability in pediatric high grade glioma is associated with ge...
Pereira, Marta Viana; Lee, Alicia; Popov, Sergey; Bax, Dorine A.; Al-Sarraj, Safa; Bridges, Leslie R.; Stávale, João N.; Jones, Chris; Reis, R. M.The authors would like to thank Dr. Steve Jackson's Laboratory at the University of Cambridge for the MRE11 antibody and Dr. Sandra Costa from ICVS, University of Minho, for the disease-free controls DNA ; High grade gliomas (HGG) are one of the leading causes of cancer-related deaths in children, and there is increasing evidence that pediatric HGG may harbor distinct molecular characteristics compared to adul...
Zigzag graphene nanoribbon edge reconstruction with stone-wales defects
Rodrigues, João N. B.; Gonçalves, P. A. D.; Rodrigues, N. F. G.; Ribeiro, R. M.; Santos, J. M. B. Lopes dos; Peres, N. M. R.In this paper, we study zigzag graphene nanoribbons with edges reconstructed with Stone-Wales defects, by means of an empirical (first-neighbor) tight-binding method, with parameters determined by ab initio calculations of very narrow ribbons. We explore the characteristics of the electronic band structure with a focus on the nature of edge states. Edge reconstruction allows the appearance of a new type of edge ...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||