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Application of flow cytometry for the identification of Staphylococcus epidermi...

Staphylococcus epidermidis is considered to be one of the most common causes of nosocomial bloodstream infections, particularly in immune-compromised individuals. Here, we report the development and application of a novel peptide nucleic acid probe for the specific detection of S. epidermidis by fluorescence in situ hybridization. The theoretical estimates of probe matching specificity and sensitivity were 89 a...

Detection of staphylococcus epidermidis on biofilms by fluorescence in situ hyb...

DNA mimics for the rapid identification of microorganisms by fluorescence in si...

Fluorescence in situ hybridization (FISH) is a well-established technique that is used for a variety of purposes, ranging from pathogen detection in clinical diagnostics to the determination of chromosomal stability in stem cell research. The key step of FISH involves the detection of a nucleic acid region and as such, DNA molecules have typically been used to probe for the sequences of interest. However, since...

Development of novel peptide nucleic acid (PNA) probes for the rapid identifica...