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Effects of molybdate and tungstate on expression levels and biochemical charact...
Mota, Cristiano S.; Valette, Odile; J. González, Pablo; Brondino, Carlos D.; Moura, José J. G.; Moura, Isabel; Dolla, Alain; Rivas, Maria G.Journal of Bacteriology. 2011 Jun; Vol. 193 issue 12 pages 2917-2923 ; Formate dehydrogenases (FDHs) are enzymes that catalyze the formate oxidation to carbon dioxide and that contain either Mo or W in a mononuclear form in the active site. In the present work, the influence of Mo and W salts on the production of FDH by Desulfovibrio alaskensis NCIMB 13491 was studied. Two different FDHs, one containing W (W...
Heterodimeric nitrate reductase (NapAB) from Cupriavidus necator H16: purificat...
Coelho, Catarina; J. Gonzaléz, Pablo; Trincão, José; Carvalho, Ana L.; Najmudin, Shabir; Moura, José J. G.; Hettman, Thomas; Dieckman, StephanActa Cryst. (2007). F63, 516–519 ; The periplasmic nitrate reductase from Cupriavidus necator (also known as Ralstonia eutropha) is a heterodimer that is able to reduce nitrate to nitrite. It comprises a 91 kDa catalytic subunit (NapA) and a 17 kDa subunit (NapB) that is involved in electron transfer. The larger subunit contains a molybdenum active site with a bis-molybdopterin guanine dinucleotide cofactor as...
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