Encontrados 4 documentos, a visualizar página 1 de 1
Two distinct arabinofuranosidases contribute to arabino-oligosaccharide degrada...
Sá-Nogueira, Isabel de; Inácio, José Manuel; Correia, Isabel LopesMicrobiology 154 (2008) 2719-2729 ; Bacillus subtilis produces alpha-l-arabinofuranosidases (EC 3.2.1.55; AFs) capable of releasing arabinosyl oligomers and l-arabinose from plant cell walls. Here, we show by insertion-deletion mutational analysis that genes abfA and xsa(asd), herein renamed abf2, encode AFs responsible for the majority of the intracellular AF activity in B. subtilis. Both enzyme activities we...
Characterization of the abn2(yxiA) encoding a Bacillus subtilis GH43 arabinanas...
Sá-Nogueira, Isabel de; Inácio, José ManuelJournal of Bacteriology (Junho 2008) 4272-4280 ; The extracellular depolymerization of arabinopolysaccharides by microorganisms is accomplished by arabinanases, xylanases, and galactanases. Here, we characterize a novel endo-alpha-1,5-l-arabinanase (EC 3.2.1.99) from Bacillus subtilis, encoded by the yxiA gene (herein renamed abn2) that contributes to arabinan degradation. Functional studies by mutational anal...
Overproduction, crystallization and preliminary X-ray characterization of Abn2,...
Sá-Nogueira, Isabel de; Sanctis, Daniele de; Bento, Isabel; Inácio, José Manuel; Custódio, Sónia; Carrondo, Maria ArméniaActa Crystallographica F64 (2008) 636-638 ; Two Bacillus subtilis extracellular endo-1,5-alpha-L-arabinanases, AbnA and Abn2, belonging to glycoside hydrolase family 43 have been identified. The recently characterized Abn2 protein hydrolyzes arabinan and has low identity to other reported 1,5-alpha-L-arabinanases. Abn2 and its selenomethionine (SeMet) derivative have been purified and crystallized. Crystals ap...
trans-Acting factors and cis elements involved in glucose repression of arabina...
Sá-Nogueira, Isabel de; Inácio, José ManuelJournal of Bacteriology (Nov 2007) 8371-8376 ; In Bacillus subtilis, the synthesis of enzymes involved in the degradation of arabinose-containing polysaccharides is subject to carbon catabolite repression (CCR). Here we show that CcpA is the major regulator of repression of the arabinases genes in the presence of glucose. CcpA acts via binding to one cre each in the promoter regions of the abnA and xsa genes a...
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