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A new lineage of halophilic, wall-less, contractile bacteria from a brine-fille...
Antunes, André; Rainey, Fred A.; Wanner, Gerhard; Taborda, Marco; Pätzold, Jürgen; Nobre, M. Fernanda; Costa, Milton S. da; Huber, RobertA novel strictly anaerobic bacterium designated strain SSD-17B(T) was isolated from the hypersaline brine-sediment interface of the Shaban Deep, Red Sea. Cells were pleomorphic but usually consisted of a central coccoid body with one or two "tentacle-like" protrusions. These protrusions actively alternated between a straight, relaxed form and a contracted, corkscrew-like one. A peptidoglycan layer was not detec...
The isolation and characterization of cytochrome c nitrite reductase subunits (...
Moura, Isabel; Almeida, Maria Gabriela; Macieira, Sofia; Gonçalves, Luisa L.; Huber, Robert; Cunha, Carlos A.; Romão, Maria J.; Costa, CristinaEur. J. Biochem. 270, 3904–3915 (2003) ; The cytochrome c nitrite reductase is isolated from the membranes of the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 as a heterooligomeric complex composed by two subunits (61 kDa and 19 kDa) containing c-type hemes, encoded by the genes nrfA and nrfH,respectively. The extracted complex has in average a 2NrfA:1NrfH composition. The separation of cc...
Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774
Moura, Isabel; Cunha, Carlos A.; Macieira, Sofia; Dias, João M.; Almeida, Gabriela; Gonçalves, Luisa L.; Costa, Cristina; Lampreia, Jorge; Huber, RobertThe Journal of Biological Chemistry Vol. 278, No. 19, Issue of May 9, pp. 17455–17465, 2003 ; The gene encoding cytochrome c nitrite reductase(NrfA) from Desulfovibrio desulfuricans ATCC 27774 was sequenced and the crystal structure of the enzyme was determined to 2.3-Å resolution. In comparison with homologous structures, it presents structural differences mainly located at the regions surrounding the putativ...
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