Background: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic inform...
Phytophthora cinnamomi Rands is involved in the decline and mortality of Quercus suber L. and Quercus ilex L. in Southern Europe, in particular in Portugal and Spain. The presence and spread of P. cinnamomi in these regions is a severe threat to these oak ecosystems leading to expectable severe consequences for the production of cork and acorns in the near future. Molecular mechanisms underlying oomycete-host i...
The virulence of two wild type (PA45 and PA37) and two genetically modified (13C: hygromycin resistant; FATSS: hygromycin resistant and β-cin knock-down) Phytophthora cinnamomi strains towards cork oak (Quercus suber) was assessed via a quantitative evaluation of disease symptoms arising from a soil infestation assay, and by a istological analysis of root colonization. Comparison of virulence, as expressed by ...
Beja – Portugal, Março de 2007 ; Encontro ; Os produtos hortofrutícolas estão frequentemente em contacto com o solo, insectos, animais e seres humanos não só durante o desenvolvimento e colheita como por vezes na indústria de processamento. Por essas razões as camadas superfíciais dos produtos podem estar expostas a contaminantes naturais, chegando a atingir 104 a 106 microrganismos por grama. Embora as bacté...
Besnoitia besnoiti impact on fertility of cattle exploited in Mediterranean pastures (Alentejo) Summary Besnoitia besnoiti is a bovine parasite endemic in many tropical and subtropical areas whose prevalence in the Mediterranean countries such as Portugal seems to be increasing. Most infections are mild or subclinical, characterized by the formation of numerous cutaneous and sub-cutaneous microcysts, lowering ...
Tese dout., Biologia, Universidade do Algarve, 2006 ; Phytophthora cinnamomi, oomiceta parasita das raízes de Quercus suber, excreta abundantemente cinamominas, proteínas da família das elicitinas. Foi demonstrada a interacção destas proteínas com vários tipos de moléculas lipídicas mas desconhece-se o seu papel biológico. Neste trabalho foi induzido o silenciamento do gene codificante para a β-cinamomina, apó...
A gene encoding a potential NADPH-dependent cinnamyl alcohol dehydrogenase (QsCAD1) (GenBank accession no: AY362455) was identified in Quercus suber (cork oak). Its complete cDNA sequence was obtained by RACE-PCR, starting from total RNA extracted from roots of seedlings of Q. suber, infected with Phytophthora cinnamomi, the causal agent of the decline and sudden death of Q. suber and Quercus ilex subsp. rotund...
Samples of 10 and 14 Ctenocephalides felis felis fleas were collected on dogs from Pedreira and Mogi das Cruzes municipalities, respectively, in the State of São Paulo, Brazil, for detection of Rickettsia spp. Individual fleas were submitted to Polymerase Chain Reaction targeting the 17-kDa and the 190-kDa (OmpA) genes of Rickettsiae. This later gene is specific for spotted fever group. Nine fleas from Pedreira...
RESUMO Todos sabemos que as crianças não são meramente pequenos adultos. Em comparação com aqueles, estas apresentam uma relação superfície cutânea/ peso corporal aumentada que contribui, por um lado, para aumentar a susceptibilidade à irritação, por outro, para maximizar a absorção percutânea com consequente aumento do risco de toxicidade sistémica. Tendo em atenção os aspectos particulares da pele da criança,...
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