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Cryopreservation of ovine in vitro produced embryos using centrifugation and cy...
Romão, Ricardo Jorge; Marques, Carla; Batista, Maria; Barbas, João; Horta, António; Carolino, Nuno; Bettencourt, Elisa; Pereira, RosaThe cryosurvival of ovine in vitro produced embryos are still low, thus preventing its routine transfer. Attempts have been made to override this problem by decreasing embryos lipid content or protecting their structure with cytoskeletal stabilizers. In this study we used embryo mechanical delipidation through centrifugation in the presence or absence of cytocalasin D testing its effect on embryo quality and cr...
Evaluation of two methods of in vitro production of ovine embryos using fresh o...
Romão, Ricardo; Marques, Carla; Batista, Maria Conceição; Vasques, Maria Irene; Barbas, J Pedro; Horta, António M; Carolino, NunoSuccessful production of high quality blastocysts depends on the use of a culture system that ensures the acquisition of developmental competence by the maturing oocyte followed by an efficient in vitro fertilization. In the present work the effect of FSH and pyruvate in an EGF containing medium for ovine oocyte maturation prior to insemination with fresh(F)or frozen–thawed (FT) semen on embryo developmental co...
Effect of arachidonic acid supplementation and cyclooxygenase/lipoxygenase inhi...
Pereira,Rosa Maria; Marques,Carla Cruz; Baptista,Maria da Conceição; Vasques,Maria Irene; Horta,António EduardoThe effect of arachidonic acid (AA) cascade on bovine embryo development in a granulosa cell co-culture system was studied. Arachidonic acid (100 µM) was supplemented from 1-cell to 8-16 cell block stage (first three days of co-culture) and from 1-cell to hatching. Specific cyclooxygenase (indomethacin, 28 µM) and lipoxygenase (nordihydroguaiaretic acid - NDGA, 28 µM) inhibitors were used from 1-cell to 8-16 ce...
Dystrophies of the abdominal wall in adults: Surgical treatment
Athias,Jayme Fortunato; Horta,António Carlos Bentes; Franco,Glaucilene Aragão; Silva,Alexandre Augusto Mekdec da; Neves,Mauro SantosThe authors present a retrospective study of 60 patients with abdominal dystrophies, both with and without hernias, operated on in the period between 01 June 1994 and 30 January 1996. Their average age was 47 years. The incision at hernia itself and the dystrophic abdomen were the principal objects of the study. The authors show the importance of abdominal dermolipectomy for the complete recovery of the patient...
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