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A PRND polymorphism in Churra do Campo portuguese sheep breed
Mesquita, P.; Silva, M.F.S.; Carolino, M.C.; Sousa, M.C.O.; Pimenta, J.; Marques, M.R.; Santos, I.C.; Gama, L.T.; Andrade, C.S.C.R.; Fontes, C.M.Prion-like Doppel gene (PRND) is located downstream from priori protein gene (PRNP). Doppel protein is not related to prion disease but to male fertility. Our previous analysis of PRND coding region in 460 animals from 8 Portuguese sheep breeds, by multiple restriction fragment-single strand conformation polymorphism (MRFSSCP), revealed a synonymous substitution (78G>A). An association was found between identif...
Besnoitia besnoiti impact on fertility of cattle exploited in mediterranean pas...
Cortes, Helder; Chagas e Silva, J.; Baptista, M.C.; Pereira, R.M.; Leitão, A.; Horta, A.E.M.; Vasques, M.I.; Barber, J.; Marques, C.C.Besnoitia besnoiti is a bovine parasite endemic in many tropical and subtropical areas whose prevalence in the Mediterranean countries such as Portugal seems to be increasing. Most infections are mild or subclinical, characterized by the formation of numerous cutaneous and sub-cutaneous microcysts, lowering the quality of skins for the leather industry. Male sterility or impaired fertility is a common sequela i...
Cyclooxygenase-2 (COX-2) expression by in vitro produced bovine embryos. Prelim...
Marques, C.C.; Horta, A.E.; Vasques, M.I.; Baptista, M.C.; Becker, J.D.; Pimenta, J.; Pereira, R.M.Expression of the two isoforms of cyclooxygenase enzyme, cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2), by in vitro produced bovine embryos was examined. Cumulus-oocyte complexes were recovered from ovaries of slaughtered animals and subsequently in vitro matured and in vitro fertilized. Presumptive zygotes were cultured in serum containing medium (TCM 199+10% bovine superovulated oestrus serum) on a gr...
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