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Identification of Li+ binding sites and the effect of Li+ treatment on phosphol...
Layden, Brian T.; Abukhdeir, Abde M.; Malarkey, Christopher; Oriti, Lisa A.; Salah, Wajeeh; Stigler, Claire; Geraldes, Carlos F. G. C.Li+ binding in subcellular fractions of human neuroblastoma SH-SY5Y cells was investigated using 7Li NMR spin-lattice (T1) and spin-spin (T2) relaxation measurements, as the T1/T2 ratio is a sensitive parameter of Li+ binding. The majority of Li+ binding occurred in the plasma membrane, microsomes, and nuclear membrane fractions as demonstrated by the Li+ binding constants and the values of the T1/T2 ratios, wh...
Evaluation of [Co(gly)3]- as a 35Cl- NMR Shift Reagent for Cellular Studies
Diven, Conrad F.; Wang, Fei; Abukhdeir, Abde M.; Salah, Wajeeh; Layden, Brian T.; Geraldes, Carlos F. G. C.; Freitas, Duarte Mota deWe studied the efficacy of the tris-glycinatocobaltate(II) complex ([Co(gly)3]-) as a shift reagent (SR) for chloride by 35Cl NMR spectroscopy and compared to that of Co2+(aq). Due to the relatively low thermodynamic stability of [Co(gly)3]-, a 1:3 Co(II)/gly stoichiometric solution at physiological pH is approximately a 2:1 mixture of [Co(gly)2(H2O)2] and [Co(gly)(H2O)4]+. This SR was found to be stable up to ...
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