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Growing pollen tubes possess a constitutive alkaline band in the clear zone and...

Using both the proton selective vibrating electrode to probe the extracellular currents and ratio-metric wide-field fluorescence microscopy with the indicator 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF)-dextran to image the intracellular pH, we have examined the distribution and activity of protons (Hf) associated with pollen tube growth. The intracellular images reveal that lily pollen tube...