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Fluorescence in situ hybridization method using peptide nucleic acid probes for...
Machado, António; Almeida, Carina; Salgueiro, D. A. L.; Henriques, Ana Filipa; Vaneechoutte, Mario; Haesebrouck, Freddy; Vieira, M. J.; Rodrigues, L. R.Background: Bacterial vaginosis (BV) is a common vaginal infection occurring in women of reproductive age. It is widely accepted that the microbial switch from normal microflora to BV is characterized by a decrease in vaginal colonization by Lactobacillus species together with an increase of Gardnerella vaginalis and other anaerobes. Our goal was to develop and optimize a novel Peptide Nucleic Acid (PNA) Fluore...
In silico vs in vitro analysis of primer specificity for the detection of Gardn...
Henriques, Ana Filipa; Cereija, Tatiana; Machado, António; Cerca, NunoBacterial vaginosis (BV) is a common pathology of women in reproductive age that can lead to serious health complications, and is associated with shifts in the normal microflora from predominance of Lactobacillus spp. to a proliferation of other anaerobes such as G. vaginalis and A vaginae, which can be detected by PCR. The optimal PCR pathogen detection assay relies mainly on the specificity and sensitivity of...
Optimizing a qPCR gene expression quantification assay for S. epidermidis biofi...
França, Ângela; Freitas, Ana I.; Henriques, Ana Filipa; Cerca, NunoStaphylococcus epidermidis biofilm-related infections are a current concern within the medical community due to their high incidence and prevalence, particularly in patients with indwelling medical devices. Biofilm gene expression analysis by quantitative real-time PCR (qPCR) has been increasingly used to understand the role of biofilm formation in the pathogenesis of S. epidermidis infections. However, dependi...
Characterization of biofilm-forming microorganisms isolated from vaginal exudat...
Alves, P.; Castro, J.; Cereija, Tatiana; Henriques, Ana Filipa; Cerca, NunoBacterial vaginosis (BV) is one of the most common gynaecological conditions affecting women in the reproductive age, and can lead to increase risk gynaecological infections and pre-term labour. The aetiology of this pathology is still poorly understood, but recent reports referring to the presence of anaerobic biofilms both in the healthy and BV vagina (with different microbial compositions) have led to the th...
Detection of Lactobacillus and Gardnerella species in vaginal samples by PNA-FISH
Machado, António; Salgueiro, Débora; Almeida, Carina; Henriques, Ana Filipa; Haesebrouck, Freddy; Vaneechoutte, Mario; Rodrigues, L. R.; Cerca, NunoBacterial vaginosis (BV) is a common vaginal infection of women in reproductive age. This infection is initially asymptomatic and late diagnosis can increase the health costs and hamper treatment. Therefore, an easy and quick method to accurately detect the transition between normal microflora to the predecessors of infection is of upmost importance. Despite the fact that BV etiology remains unknown, it is comm...
Presence of Gardnerella vaginalis in healthy portuguese women : a pilot study
Salgueiro, D. A. L.; Machado, António; Alves, Patrícia; Oliveira, José Martinez de; Henriques, Ana Filipa; Cerca, NunoBacterial vaginosis (BV) has an important position worldwide, as the leading vaginal disorder in women, and affects 30-50% of African women and 10-20% of White women of reproductive age. This condition although not mortal causes great discomfort and may lead to other complications such as pre-term labour or increase susceptibility for HIV infection. During BV occur a decrease of Lactobacillus spp. present in t...
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