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Enzymatic reduction of azo and indigoid compounds
Pricelius, S.; Held, C.; Murkovic, M.; Bozic, M.; Kokol, V.; Paulo, Artur Cavaco; Gübitz, Georg M.A customer- and environment-friendly method for the decolorization azo dyes was developed. Azoreductases could be used both to bleach hair dyed with azo dyes and to reduce dyes in vat dyeing of textiles. A new reduced nicotinamide adenine dinucleotide-dependent azoreductase of Bacillus cereus, which showed high potential for reduction of these dyes, was purified using a combination of ammonium sulfate precipita...
Enzymatic reduction and oxidation of fibre-bound azo-dyes
Pricelius, S.; Held, C.; Sollner, S.; Deller, S.; Murkovic, M.; Ullrich, R.; Hofrichter, M.; Paulo, Artur Cavaco; Macheroux, P.; Guebitz, G. M.A new customer and environmental friendly method of hair bound dye decolouration was developed. Biotransformation of the azo-dyes Flame Orange and Ruby Red was studied using different oxidoreductases. The pathways of azo dye conversion by these enzymes were investigated and the intermediates and metabolites were identified and characterised using UV–vis spectroscopy, high-performance liquid chromatography (HPLC...
Biotransformation of phenolics with laccase containing bacterial spores
Held, C.; Kandelbauer, A.; Schroeder, M.; Paulo, Artur Cavaco; Gübitz, Georg M.Treatment of effluents containing phenols such as textile dyes with fungal laccases is usually limited to the acid to neutral pH range and moderate temperatures. Here we demonstrate for the first time that spore-bound laccases which are stable at high temperatures and pH values can be used for phenolic dye decolorisation. Laccase containing spores from Bacillus SF were immobilized on alumina pellets. Both immob...
Biotransformation of phenolics with laccase containing bacterial spores
Held, C.; Kandelbauer, A.; Schroeder, M.; Paulo, Artur Cavaco; Gübitz, Georg M.Treatment of effluents containing phenols such as textile dyes with fungal laccases is usually limited to the acid to neutral pH range and moderate temperatures. Here we demonstrate for the first time that spore-bound laccases which are stable at high temperatures and pH values can be used for phenolic dye decolorisation. Laccase containing spores from Bacillus SF were immobilized on alumina pellets. Both immob...
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