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Multiple gene expression by chromosomal integration and CRE-loxP mediated marke...

The level of glucose-6-phosphate dehydrogenase activity strongly influences xyl...

Disruption of the ZWF1 gene encoding glucose-6-phosphate dehydrogenase (G6PDH) has been shown to reduce the xylitol yield and the xylose consumption in the xylose-utilizing recombinant Saccharomyces cerevisiae strain TMB3255. In the present investigation we have studied the influence of different production levels of G6PDH on xylose fermentation. We used a synthetic promoter library and the copper...

Effect of enhanced xylose reductase activity on xylose consumption and product ...

Recombinant Saccharomyces cerevisiae TMB3001, harboring the Pichia stipitis genes XYL1 and XYL2 (xylose reductase and xylitol dehydrogenase, respectively) and the endogenous XKS1(xylulokinase), can convert xylose to ethanol. About 30% of the consumed xylose, however, is excreted as xylitol. Enhanced ethanol yield has previously been achieved by disrupting the ZWF1 gene, encoding glucose-6- phosph...

The non-oxidative pentose phosphate pathway controls the fermentation rate of x...

Saccharomyces cerevisiae is able to ferment xylose,when engineered with the enzymes xylose reductase (XYL1) and xylitol dehydrogenase (XYL2). However,xylose fermentation is one to two orders of magnitude slower than glucose fermentation. S. cerevisiae has been proposed to have an insufficient capacity of the non-oxidative pentose phosphate pathway (PPP) for rapid xylose fermentatio...