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Fluorescence in situ hybridization method using a peptide nucleic acid probe fo...
Machado, António; Almeida, Carina; Carvalho, Ana; Boyen, Filip; Haesebrouck, Freddy; Rodrigues, L. R.; Cerca, Nuno; Azevedo, N. F.Lactobacillus species constitute one of the dominant and beneficial bacteria in our body and are used in developed countries as a microbial adjuvant. Identification of these probiotic bacteria is traditionally performed by culture-based techniques. However, such methods are very time-consuming and can give inaccurate results, especially when Lactobacillus is present in mixed bacterial complex communities. Our s...
Fluorescence in situ hybridization method using peptide nucleic acid probes for...
Machado, António; Almeida, Carina; Salgueiro, D. A. L.; Henriques, Ana Filipa; Vaneechoutte, Mario; Haesebrouck, Freddy; Vieira, M. J.; Rodrigues, L. R.Background: Bacterial vaginosis (BV) is a common vaginal infection occurring in women of reproductive age. It is widely accepted that the microbial switch from normal microflora to BV is characterized by a decrease in vaginal colonization by Lactobacillus species together with an increase of Gardnerella vaginalis and other anaerobes. Our goal was to develop and optimize a novel Peptide Nucleic Acid (PNA) Fluore...
Detection of Lactobacillus and Gardnerella species in vaginal samples by PNA-FISH
Machado, António; Salgueiro, Débora; Almeida, Carina; Henriques, Ana Filipa; Haesebrouck, Freddy; Vaneechoutte, Mario; Rodrigues, L. R.; Cerca, NunoBacterial vaginosis (BV) is a common vaginal infection of women in reproductive age. This infection is initially asymptomatic and late diagnosis can increase the health costs and hamper treatment. Therefore, an easy and quick method to accurately detect the transition between normal microflora to the predecessors of infection is of upmost importance. Despite the fact that BV etiology remains unknown, it is comm...
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