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Inquéritos de satisfação: Triagem de Manchester no Hospital de Santo António

On the routes of Helicobacter pylori transmission among the humans

Tese de doutoramento em Engenharia Química Biológica ; Helicobacter pylori is a spiral, microaerophilic, Gram-negative bacterium that colonizes the human stomach and has been associated with the pathogenesis of chronic gastritis, peptic ulcer disease and gastric carcinoma. Since the isolation of H. pylori, numerous studies have been published addressing the prevalence and epidemiology of the infection, the rel...

Helicobacter pylori colonization of the adenotonsillar tissue : fact or fiction?

Objective: The transmission of the gastric pathogen Helicobacter pylori involves the oral route. Molecular techniques have allowed the detection of H. pylori DNA in samples of the oral cavity, although culture of H. pylori from these type of samples has been sporadic. Studies have tried to demonstrate the presence of H. pylori in adenotonsillar tissue, with contradictory results. Our aim was to clarify whether ...

H. pylori colonization of the adenotonsillar tissue : fact or fiction?

Gastric infection with Helicobacter pylori is the most common chronic infection worldwide. One of the routes of transmission of the infection is the oral route. Molecular techniques have allowed the detection of H. pylori DNA in samples of the oral cavity, although culture of H. pylori from these types of samples has been sporadic. Studies have tried to demonstrate the presence of H. pylori in adenotonsillar ti...

Water-exposed H.pylori presents decreased virulence properties

Helicobacter pylori transmission has been associated in epidemiological studies with water. H. pylori has been identified in this environment using molecular techniques. As water may be an environmental reservoir for H. pylori, and because there is lack of information regarding the capacity of water-exposed bacteria to induce a response in host cells, we assessed the cultivability of water-exposed H. pylori and...

Development and application of a novel peptide nucleic acid probe for the speci...

In this work, a fluorescence in situ hybridization (FISH) method for the rapid detection of Helicobacter pylori using a novel peptide nucleic acid (PNA) probe is reported. Laboratory testing with several different bacterial species, including other Helicobacter spp., has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection o...

A new model for the transmission of Helicobacter pylori : role of environmental...

Twenty-five years after the first successful cultivation and isolation of Helicobacter pylori, the scientific community is still struggling to understand the way(s) this bacterium is transmitted among the human population. Here, both epidemiologic and microbiologic evidence addressing this matter is reviewed and explored to conclude that most H. pylori successful colonizations are derived from direct person-to-...

A multiroute way of transmission for Helicobacter pylori

Biofilms as possible microenvironments for H. pylori subsistence in water-expos...

Development and application of a novel peptide nucleic acid (PNA) probe for the...