The objective of this work was to isolate and characterize tannin-tolerant ruminal bacteria from crossbred Holstein x Zebu cows fed a chopped mixture of elephant grass (Pennisetum purpureum), young stems of "angico-vermelho" (Parapiptadenia rigida), and banana tree (Musa sp.) leaves. A total of 117 bacteria strains were isolated from enrichment cultures of rumen microflora in medium containing tannin extracts. ...
Dissertação (mestrado)–Universidade de Brasília, Instituto de Artes, 2010. ; A presente dissertação trata de uma pesquisa de caráter teórico/prático e se deu a partir da realização do projeto artístico do Grupo Mesa de Luz, formado pelos artistas plásticos Hieronimus do Vale, Tomás Seferin e Marta Mencarini. A pesquisa partiu de uma descrição dos processos de construção do trabalho realizado pelo grupo com o...
The main purpose of the present study was to identify for candidate genes related to ovulation in swines. To do so, it was investigated in ovarian follicular cells through quantitative real-time PCR the differential expression of the following genes: steroidogenic acute regulator (STAR), GATA-binding protein 4 (GATA), prostaglandin F2α (PGF2α), progesterone receptor (P4R), follicle-stimulating hormone...
O mercado alimentar oferece um número crescente de bebidas com teores variáveis de açúcar, aditivos e estabilizadores. O consumidor actual, cada vez mais informado e preocupado com a saúde procura alternativas mais naturais e saudáveis. A indústria tem procurado dar resposta às expectativas do consumidor actual oferecendo novos produtos com menos açúcar e com características mais próximas dos produtos frescos. ...
A retrospective study was conducted to identify the occurrence and types of ocular disorders in 57 Amazon parrots admitted to the Ophthalmology Service, Veterinary Teaching Hospital, School of Veterinary Medicine, University of São Paulo, Brazil from 1997 to 2006. The most frequent observed disorder was cataracts, present in 24 of the 114 examined eyes (57 parrots). Uveitis, ulcerative keratitis and keratoconju...
In order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three...
The increasing presence of products derived from genetically modified (GM) plants in human and animal diets has led to the development of detection methods to distinguish biotechnology-derived foods from conventional ones. The conventional and real-time PCR have been used, respectively, to detect and quantify GM residues in highly processed foods. DNA extraction is a critical step during the analysis process. S...
O objetivo deste trabalho foi avaliar a associação dos polimorfismos dos genes bGH, IGF-1 e PIT-1 com características de peso e ganho de peso numa população F2 de bovinos (Gir x Holandês), pela técnica de PCR-RFLP. As freqüências alélicas A e B, do gene PIT-1, e dos genótipos AA, AB e BB, nas populações parentais foram semelhantes entre si, mas diferentes das freqüências nas populações cruzadas F1 e F2, para es...
The effect of the bovine major histocompatibility complex (BoLA) locus on animal health may be due to a direct action of its alleles on immune functions, whereas its indirect effect on production traits might be explained by the better general health conditions of more productive animals. In the present study, the BoLA-DRB3 gene was investigated in 1058 cows belonging to seven Brazilian Gyr Dairy herds (Bos ind...
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