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Geomorphological settings and tufa models in Algibre flexure (Algarve, Portugal)
Guerreiro, Paulo; Cunha, Lúcio; Ribeiro, CarlosINTRODUCTION Percolating water through organically enriched soil, raises the water pCO2 promoting an increase in carbonates dissolution. Following subaerial exposure, the water-atmosphere interaction, leads to an oversaturation of water CaCO3 and precipitation (PENTECOST, 2005; CHAFTEZ & FOLK, 1984). This precipitation can be inorganic, biologic or both (PEDLEY, 2009). The tufa facies presented in this work, fo...
INTERPRETAÇÃO GEOMORFOLÓGICA DA RIBEIRA DAS MERCÊS E AFLORAMENTOS DE TUFO CALCÁ...
Guerreiro, Paulo; Cunha, Lúcio; Ribeiro, CarlosA área da flexura de Algibre apresenta-se carsificada, sendo uma área de recarga para os aquíferos formados no seu interior que, em alguns casos estão conectados com os da área envolvente. Aqui, as superfícies aplanadas e o carso nu e subcutâneo das formas de relevo mais salientes são importantes para a recarga de um aquífero cujas águas têm tendência incrustante. Uma recarga eficaz é importante para a manutenç...
Os tufos calcários das áreas de Estói, Loulé e ribeira das Mercês (Algarve, Por...
Guerreiro, Paulo; Cunha, Lúcio; Ribeiro, Carlos; Candeias, A.In this paper we’ll define de main facies groups from the Central Algarve range calcite tufa, articulating them to the potential sedimentation palaeoenvironments. The main group of facies characterized are clastic facies (conglomerate and dusty tufa) and the accretionary (bioherm tufa), so that the first are the early and erosive stages while the latter can be attributed to the accretionary calcite tufa stages....
Os tufos calcários das áreas de Estói, Loulé e ribeira das Mercês (Algarve, Por...
Guerreiro, Paulo; Cunha, Lucio; Ribeiro, CarlosNesta comunicação tentaremos estabelecer os principais tipos de fácies apresentadas pelos tufos desta área do Algarve Central, articulando os mesmos com os potenciais paleoambientes de formação. Os principais tipos de fácies identificadas correspondem a fácies detríticas (conglomerado e tufos pulverulentos) e de optimum de acumulação (tufo biohérmico), sendo que os primeiros podem corresponder às fases iniciais...
Expression of YAP4 in Saccharomyces cerevisiae under osmotic stress
Nevitt, Tracy; Pereira, Jorge; Azevedo, Dulce; Guerreiro, Paulo; Rodrigues-Pousada, ClaudinaYAP4, a member of the yeast activator protein (YAP) gene family, is induced in response to osmotic shock in the yeast Saccharomyces cerevisiae. The null mutant displays mild and moderate growth sensitivity at 0.4 M and 0.8 M NaCl respectively, a fact that led us to analyse YAP4 mRNA levels in the hog1 (high osmolarity glycerol) mutant. The data obtained show a complete abolition of YAP4 gene expression in this ...
Disruption and phenotypic analysis of six open reading frames from chromosome V...
Guerreiro, Paulo; Rodrigues-Pousada, ClaudinaSix open reading frames (ORFs) located on chromosome VII of Saccharomyces cerevisiae (YGR205w, YGR210c, YGR211w, YGR241c, YGR243w and YGR244c) were disrupted in two different genetic backgrounds using short-flanking homology (SFH) gene replacement. Sporulation and tetrad analysis showed that YGR211w, recently identified as the yeast ZPR1 gene, is an essential gene. The other five genes are non-essential, and no...
Analysis of a 17.9 kb region from Saccharomyces cerevisiae chromosome VII revea...
Feroli, Fiorella; Carignani, Giovanna; Pavanello, Anna; Guerreiro, Paulo; Azevedo, Dulce; Rodrigues-Pousada, Claudina; Melchioretto, PasqualeWe report the nucleotide sequence of a 17,893 bp DNA segment from the right arm of Saccharomyces cerevisiae chromosome VII. This fragment begins at 482 kb from the centromere. The sequence includes the BRF1 gene, encoding TFIIIB70, the 5' portion of the GCN5 gene, an open reading frame (ORF) previously identified as ORF MGA1, whose translation product shows similarity to heat-shock transcription factors and fiv...
Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals...
Guerreiro, Paulo; Azevedo, Dulce; Barreiros, Tânia; Rodrigues-Pousada, ClaudinaA 9.9 kb DNA fragment from the right arm of chromosome VII of Saccharomyces cerevisiae has been sequenced and analysed. The sequence contains four open reading frames (ORFs) longer than 100 amino acids. One gene, PFK1, has already been cloned and sequenced and the other one is the probable yeast gene coding for the beta-subunit of the succinyl-CoA synthetase. The two remaining ORFs share homology with the deduc...
Sequencing of a 17.6 kb segment on the right arm of yeast chromosome VII reveal...
Guerreiro, Paulo; Barreiros, Tânia; Soares, Helena; Cyrne, Luísa; Maia e Silva, Alexandra; Rodrigues-Pousada, ClaudinaA 17.6 kb DNA fragment from the right arm of chromosome VII of Saccharomyces cerevisiae has been sequenced and analysed. The sequence contains twelve open reading frames (ORFs) longer than 100 amino acids. Three genes had already been cloned and sequenced: CCT, ADE3 and TR-I. Two ORFs are similar to other yeast genes: G7722 with the YAL023 (PMT2) and PMT1 genes, encoding two integral membrane proteins, and G772...
The Tetrahymena chaperonin subunit CCT eta gene is coexpressed with CCT gamma g...
Cyrne, Luísa; Guerreiro, Paulo; Cardoso, Ana Cristina; Rodrigues-Pousada, Claudina; Soares, HelenaWe report here the cloning and the characterization of the T. pyriformis CCT eta gene (TpCCT eta) and also a partial sequence of the corresponding T. thermophila gene (TtCCT eta). The TpCCt eta gene encodes a protein sharing a 60.3% identity with the mouse CCT eta. We have studied the expression of these genes in Tetrahymena exponentially growing cells, cells regenerating their cilia for different periods and d...
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