Encontrados 8 documentos, a visualizar página 1 de 1
Understanding SaPI1 specific packaging determinants
Bento, Joana CaetanoTese de doutoramento Programa Doutoral em Engenharia Biomédica ; Staphylococcus aureus é um agente patogénico capaz de causar uma variedade de infeções, devido à sua capacidade de expressar uma grande diversidade de fatores de virulência. S. aureus está associado a infecções adquiridas no ambiente hospitalar mas também na comunidade. Muitas das toxinas e genes virulentos que S. aureus codifica estão localizado...
Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH
Almeida, Carina; Azevedo, N. F.; Bento, Joana C.; Cerca, Nuno; Ramos, H.; Vieira, M. J.; Keevil, C. W.We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2–99.7) and 100 % (95 % CI, 80,8–100), respectively, when compared with CHROMagar Orientation medium. Results ind...
Farnesol induces cell detachment from established S. epidermidis biofilms
Cerca, Nuno; Gomes, F. I.; Bento, Joana C.; França, Ângela; Rolo, Joana; Miragaia, Maria; Teixeira, P.; Oliveira, RosárioAntibiotic resistance is a serious problem in Staphylococcus epidermidis infections as many clinical isolates of this organism are resistant to up to eight different antibiotics. The increased resistance to conventional antibiotic therapy has lead to the search for new antimicrobial therapeutic agents. Farnesol, an essential oil found in many plants, has been shown to be active against S. epidermidis. Using a t...
As conceções dos alunos do 1.º Ciclo do Ensino Básico sobre a Roda dos Alimentos
Felizardo, Diana; Bento, Joana; Noné, Laura; Conde, Micaela; Cavadas, BentoEste trabalho de investigação foi desenvolvido no âmbito da unidade curricular de Investigação em Estudo do Meio da Licenciatura em Educação Básica da Escola Superior de Educação do Instituto Politécnico de Santarém. Os seus principais objetivos foram identificar as conceções dos alunos do 1.º CEB sobre a roda dos alimentos, nomeadamente a quantidade de fatias que conhecem e a sua constituição, dando especial a...
Variability of RNA quality extracted from biofilms of foodborne pathogens using...
França, Ângela; Bento, Joana C.; Cerca, NunoThe biofilm formation by foodborne pathogens is known to increase the problem related with surface disinfection procedure in the food processing environment and consequent transmission of these pathogens into the population. Messenger RNA has been increasingly used to understand the action and the consequences of disinfectants in the virulence on such biofilms. RNA quality is an important requirement for any RN...
Farnesol antimicrobial role as biofilm cell detachment inducer in S. epidermidi...
Cerca, Nuno; Gomes, F. I.; França, Ângela; Bento, Joana C.; Rolo, Joana; Miragaia, Maria; Teixeira, P.; Vilanova, Manuel; Oliveira, RosárioObjetives: Farnesol is a naturally-occurring sesquiterpene that was originally isolated from essential oils found in many plants has been described to have antimicrobial potential against several bacteria, including S. epidermidis. However, farnesol mechanism of action is not yet fully understood and some contradictory findings have been reported. We recently described that while farnesol was not efficient at k...
Development of a rapid method for Proteus spp. detection in urine by peptide nu...
Almeida, Carina; Azevedo, N. F.; Cerqueira, L.; Bento, Joana C.; Vieira, M. J.; Keevil, C. W.The need to avoid empirical treatment of patients, and the fact that urine samples are among the most numerous of specimen types sent for microbiology studies, have prompted many researchers to explore methods to limit the time and expense of urine culture processing. The aim of this work was to develop a new peptide nucleic acid fluorescence in situ hybridization (PNA FISH) method for the rapid detection of Pr...
Doseamento das granzimas A e B na sarcoidose pulmonar (estudo experimental)
Dourado, Marília; Bento, Joana; Mesquita, Luis; Marques, Alcide; Vale-Pereira, Sofia; Sarmento-Ribeiro, Ana Bela; Mota-Pinto, AnabelaSarcoidosis is a systemic disease of unknown aetiology, morphologically characterized by well-formed epithelioid granulomas, which show little or no central necrosis. These may be present in any organ or tissue. The lung is the most frequently and prominently involved target. The granuloma is often very sharply demarcated from the adjacent tissue and is surrounded by a mantle of lymphocytes, which mediate lysis...
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