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In vitro flowering and viable seed setting of transgenic lettuce cultures

Gregory, Franklin; Oliveira, Ana Lucia; Dias, Alberto Carlos Pires

Production of transgenic lettuce seeds via in vitro flowering and fruit setting is reported here. Six days old cotyledons were co-cultivated with Agrobacterium tumefaciens strain EHA105 harbouring the binary vector pCAMBIA2301 carrying the reporter gene a-glucuronidase intron (GUS-INT) and the marker gene neomycin phosphotransferase (NPTII). Transgenic calluses and shoot buds were induced on MS medium augmented ...


Transgenic hypericum perforatum

Gregory, Franklin; Oliveira, Margarida M.; Dias, Alberto Carlos Pires

Plant transformation is an important tool with many applications in modern plant biology. Although this technique is primarily used to produce superior crop varieties, it is also being utilized to answer basic questions concerning gene function and regulation in contemporary functional genomics research. In our laboratory, we have established a transformation system for Hypericum perforatum . This protocol invo...


Poly(D,L-lactic-co-glycolic acid) nanoencapsulation reduces erlotinib-Induced s...

Gregory, Marslin; Sheeba, Caroline J.; Kalaichelvan, V. K.; Manavalan, R.; Reddy, Neelakanta; Gregory, Franklin

Erlotinib-HCl is a quinazoline derivative used as a drug in the therapy of non-small-cell lung cancer. The present study was conducted to compare the subacute toxicity induced by Erlotinib-HCl delivered to rats as nanoparticles and as free drug. Wistar rats were orally administered with a daily dosage of 200 mg kg−1 Erlotinib-HCl either as free drug or as Poly(D,L-lactic-co-glycolic acid) (PLGA) encapsulated na...


Anti-ulcer (ulcer-preventive) activity of ficus arnottiana miq. (moraceae) leaf...

Gregory, Marslin; Vithalrao, K. P.; Gregory, Franklin; Kalaichelvan, V. K.

Problem statement: In spite of being one of the well-known medicinal plants used in Indian traditional medicine to treat several ailments, studies pertaining to the pharmacological properties of Ficus arnottiana are very scarce. We studied the anti-ulcer activity and acute toxicity of Ficus arnottiana leaf methanolic extract for the first time. Approach: Freshly collected F. arnottiana leaves were dried, powder...


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Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia