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Mineral stress affects the cell wall composition og grapevine (Vitis vinifera L...
Fernandes, João C.; Garcia-Angulo, Penélope; Goulão, Luis F.; Acebes, José L.; Amâncio, SaraVersão provisória aceite p. publicação na Plant Science (ISSN 0168-9452)em 19-01-2013 ; Grapevine (Vitis vinifera L.) is one of the most economically important fruit crops in the world. Deficit in nitrogen, phosphorus and sulfur nutrition impairs essential metabolic pathways. The influence of mineral stress in the composition of the plant cell wall (CW) has received residual attention. Using grapevine callus a...
Efeito do 1-metilciclopropeno em maçãs 'Royal Gala' armazenadas em atmosfera no...
Oliveira, Cristina Moniz; Dionísio, Luís; Neto, Cláudia; Goulão, Luís; Clemente, Josué; Henriques, Jorge; Lourenço, InêsIn order to study the evolution of the quality characteristics of apples cv. Royal Gala under refrigerated air storage, 625 ppb of 1-methylcyclopropene (1-MCP) were applied to the fruits for 24 h at 0 ºC. Fruit samples were analysed after storage and after 7 days at 20 ºC (shelf-life) for titratable acidity, pulp firmness, total soluble solids content and skin colour along a 180 days storage period. The storage...
Efeito do 1-metilciclopropeno em pera 'Rocha' em diferentes estados de maturaçã...
Oliveira, Cristina Moniz; Alpalhão, André; Neto, Cláudia; Goulão, Luís; Clemente, Josué; Henriques, Jorge; Lourenço, InêsThe effect of 1-methylcyclopropene (1-MCP) treatment on the ripening of ‘Rocha’ pears harvested with different stages of maturity, during a cold storage period of seven months was evaluated. Twenty four hours after harvest, the fruits were treated with 300 ppb of 1-MCP for 24 h at 0 ºC. The fruits were stored under normal atmosphere at 0 ºC and 90-95 % RH. Fruit samples were removed from storage periodically an...
Cell wall modifications during fruit ripening: when a fruit is not the fruit
Goulão, Luis F.; Oliveira, Cristina M.Textural changes that lead to softening of fruits are accompanied by loss of neutral sugars, solubilisation and depolymerisation of the polysaccharides of the cell wall, and rearrangements of their associations, as the result of the combined action of several cell wall-modifying enzymes, acting in both pectic and hemicellulosic fractions. Recent studies on the structure of the plant cell wall have disclosed a l...
Cloning, characterisation and expression analysis of cDNA clones encoding cell ...
Goulão, Luis F.; Cosgrove, Daniel J.; Oliveira, Cristina M.Fruit softening is accompanied by modifications of the cell wall pectic and hemicellulosic fractions, as the result of the combined action of several cell wall-modifying enzymes. The objective of this work was to clone specific cDNAs that encode isoforms of cell wall-modifying enzymes, which are expressed during the final stages of apple softening, and to establish a temporal sequence of their accumulation. A c...
Patterns of enzymatic activity of cell wall-modifying enzymes during growth and...
Sousa, Isabel; Goulao, Luis F.; Santos, João; Oliveira, Cristina MonizFruit softening is thought to result from extensive cellwall modifications that occur during ripening. These modifications are the result, at least in part, of the activity of members of cell wall-modifying enzymes from the same families involved in the cell wall loosening which promote tissue extension and growth. In this work, the activities of a set of pectolytic and non-pectolytic cell wall-modifying enzyme...
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