Encontrados 9 documentos, a visualizar página 1 de 1
Identification of cell-surface mannans in a virulent Helicobacter pylori strain
Ferreira, José Alexandre; Azevedo, N. F.; Vieira, M. J.; Figueiredo, C.; Goodfellow, Brian J.; Monteiro, Mário A.; Coimbra, Manuel A.With the intent of contributing to a carbohydrate-based vaccine against the gastroduodenal pathogen, Helicobacter pylori, we report here the structure of cell-surface mannans obtained from a virulent strain. Unlike other wild-type strains, this strain was found to express in good quantities this polysaccharide in vitro. Structural analysis revealed a branched mannan formed by a backbone of α-(1→6)-linked mannop...
An NMR structural study of nickel-substituted rubredoxin
Goodfellow, Brian J.; Duarte, Iven C. N.; Macedo, Anjos L.; Volkman, Brian F.; Nunes, Sofia G.; Moura, Isabel; Markley, John L.; Moura, José J. G.J Biol Inorg Chem (2010) 15:409–420 DOI 10.1007/s00775-009-0613-6 ; The Ni(II) and Zn(II) derivatives of Desulfovibrio vulgaris rubredoxin (DvRd) have been studied by NMR spectroscopy to probe the structure at the metal centre. The βCH2 proton pairs from the cysteines that bind the Ni(II) atom have been identified using 1D nuclear Overhauser enhancement (NOE) difference spectra and sequence specifically assign...
Metabolite Profiling of Human Amniotic Fluid by Hyphenated Nuclear Magnetic Res...
Graça, Gonçalo; Duarte, Iola F.; Goodfellow, Brian J.; Carreira, Isabel M.; Couceiro, Ana Bela; Domingues, Maria do Rosário; Spraul, ManfredThe metabolic profiling of human amniotic fluid (HAF) is of potential interest for the diagnosis of disorders in the mother or the fetus. In order to build a comprehensive metabolite database for HAF, hyphenated NMR has been used, for the first time, for systematic HAF profiling. Experiments were carried out using reverse-phase (RP) and ion-exchange liquid chromatography (LC), in order to detect less and more p...
Metabolic characterisation of plasma in juveniles with glycogen storage disease...
Duarte, Iola F.; Goodfellow, Brian J.; Barros, António; Jones, John G.; Barosa, Cristina; Diogo, Luisa; Garcia, Paula; Gil, Ana M.This paper reports the first application of high-resolution 1H NMR spectroscopy to the plasma of five juveniles with glycogen storage disease type 1a (GSD1a), permitting the characterisation of the plasma metabolic profile and the identification of alterations relative to a set of control samples. The relaxation-weighted spectra allowed changes in low molecular weight compounds to be detected more clearly, wher...
In Situ Synthesis of Magnetite Nanoparticles in Carrageenan Gels
Daniel-da-Silva, Ana L.; Trindade, T.; Goodfellow, Brian J.; Costa, Benilde F. O.; Correia, Rui N.; Gil, Ana M.Magnetite nanoparticles have been successfully synthesized in the presence of carrageenan polysaccharides using an in situ coprecipitation method. Iron coordination to the sulfate groups of the polysaccharide was confirmed by FTIR. The polysaccharide type (κ, ι, or λ) and concentration have been varied and their effects on particle morphology and chemical stability of the resultant nanocomposite investigated. T...
In situ synthesis of magnetite nanoparticles in carrageenan gels
Daniel-da-Silva, Ana. L.; Trindade, T.; Goodfellow, Brian J.; Costa, Benilde F. O.; Correia, Rui N.; Gil, Ana M.Magnetite nanoparticles have been successfully synthesized in the presence of carrageenan polysaccharides using an in situ coprecipitation method. Iron coordination to the sulfate groups of the polysaccharide was confirmed by FTIR. The polysaccharide type ( , é, or ì) and concentration have been varied and their effects on particle morphology and chemical stability of the resultant nanocomposite investigated. T...
Morphology and miscibility of chitosan/soy protein blended membranes
Silva, S. S.; Goodfellow, Brian J.; Benesch, Johan; Rocha, J.; Mano, J. F.; Reis, R. L.A physico-chemical characterization of blended membranes composed by chitosan and soy protein has been carried out in order to probe the interactions that allow membranes to be formed from these biopolymer mixtures. These membranes are developed aiming at applications in wound healing and skin tissue engineering scaffolding. The structural features of chitosan/soy blended membranes were investigated by means of...
Desulfovibrio gigas ferredoxin II: redox structural modulation of the [3Fe–4S] ...
Rodrigues, Pedro M.; Macedo, Anjos L.; Moura, Isabel; Moura, José J. G.; Goodfellow, Brian J.J Biol Inorg Chem (2006) 11: 307–315 DOI 10.1007/s00775-005-0077-2 ; Desulfovibrio gigas ferredoxin II (DgFdII) is a small protein with a polypeptide chain composed of 58 amino acids, containing one Fe3S4 cluster per monomer. Upon studying the redox cycle of this protein, we detected a stable intermediate (FdIIint) with four 1H resonances at 24.1, 20.5, 20.8 and 13.7 ppm. The differences between FdIIox and FdI...
Zinc-substituted desulfovibrio gigas desulforedoxins
Moura, Isabel; Goodfellow, Brian J.; Nunes, Sofia G.; Rusnak, Frank; Ascenso, Carla; Moura, José J. G.; Volkman, Brian F.; Markley, John L.Protein Science (2002), 11:2464–2470 ; Desulfovibrio gigas desulforedoxin (Dx) consists of two identical peptides, each containing one [Fe-4S]center per monomer. Variants with different iron and zinc metal compositions arise when desulforedoxin is produced recombinantly from Escherichia coli. The three forms of the protein, the two homodimers [Fe(III)/Fe(III)]Dx and [Zn(II)/Zn(II)]Dx, and the heterodimer [Fe(I...
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