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Isolation and analysis of lip2 gene from Trichoderma harzianum
Vaz, Madalena; Belo, Hélio; Jorge, Lurdes; Gonzalez, Francisco J.; Monte, Enrique; Choupina, AltinoPTDC/AGR-AAM/67628/2006 - dentification, characterization and role of molecular factors associated with the mechanisms of infection of Fagaceae species by Phytophthora cinnamomi
Isolation and analysis of lip2 gene from Trichoderma harzianum
Vaz, Madalena; Belo, Hélio; Jorge, Lurdes; Gonzalez, Francisco J.; Monte, Enrique; Choupina, AltinoPTDC/AGR-AAM/67628/2006 - Identification, characterization and role of molecular factors associated with the mechanisms of infection of Fagaceae species by Phytophthora cinnamomi ; Trichodorma spp. covers a group of fungi extremely common in soils of all climatic areas. These fungi arc efficient enzyme producers, with industrial applications, or in nature, involved in the degradation of the coli wall of the ph...
Identification of Trichoderma harzianum lip2 gene by HE-TAIL PCR
Jorge, Lurdes; Gonzalez, Francisco J.; Monte, Enrique; Choupina, AltinoTrichoderma harzianum is a widespread soil fungus, known as a biocontrol agent against soilborne plant pathogens. Its biological control against plant fungi pathogens is based on different mechanisms, namely the production of several lytic enzymes. In T. harzianum several glucanases, celulases, chitinases and proteases has been identified but little is known about its lipolytic system. The aim of this work was ...
Identification of Trichoderma harzianum lip2 gene by HE-TAIL PCR
Jorge, Lurdes; Monte, Enrique; Gonzalez, Francisco J.; Choupina, AltinoTrichoderma harzianum is a widespread soil fungus, known as a biocontrol agent against soil borne plant pathogens. Its biological control against plant fungi pathogens is based on different mechanisms, namely the production of several lytic enzymes. In T. harzianum several glucanases, cellulases, chitinases and proteases, has been identified but little is known about its lipolytic system. The aim of this work ...
HE-TAIL PCR, a powerfull tool for identification of Trichoderma harzianum lip1 ...
Jorge, Lurdes; Gonzalez, Francisco J.; Monte, Enrique; Choupina, AltinoSelected comunication. Oral presentation. ; Trichoderma harzianum is a widespread soil fungus, known as a biocontrol agent against soilborne plant pathogens. Species of Trichoderma are commercially applied as biological control agents against plant fungal pathogens based on different mechanisms, such as antibiosis, competition for nutrients and mycoparasitism. One of the mechanisms involved is the production o...
Lipase assay in yarrowia lipolytica
Morín, M.; Gonzalez, Francisco J.; Choupina, Altino; Burguillo, Francisco J.; Dominguez, ÁngelLipases (triacylglycerol acyl hydrolases, EC 3. 1.1.3) are key enzymes in fat metabolism produced by microorganisms, plants, and animals that catalyze the breakdown of triacylglycerols to free fatty adds and glycerol. In Y. lipolytica severaJ genes encoding lipases have been isoiated,LlP2 coding for a secreted lipase (Pignede et al. 1000) and LlPl and LIP3 encoding two lipase genes of the carboxylesrerase famil...
Producción de lipasas por la levadura Yarrowia lipolytica
Morín, M.; Choupina, Altino; Ferminan, E.; Burguillo, Francisco J.; Gonzalez, Francisco J.; Ruiz, L.; Dominguez, ÁngelYarrowia lipolytica es una levadura dimórfica que secreta gran cantidad de proteínas al medio de cultivo, entre ellas: proteasas - alcalinas, neutras y ácidas -, ribonucleasas, fosfatasas y lipasas. Las lipasas son enzimas importantes en el metabolismo de ácidos grasos, catalizando la hidrolisis de triglicéridos a ácidos grasos y glicerol.
The lipase system of Yarrowia lipolytica
Choupina, Altino; Gonzalez, Francisco J.; Morín, M.; Burguillo, Francisco J.; Ferminan, E.; Dominguez, ÁngelAmong yeast species, Yarrowia lipolytica is one of the highest producers of extracellular proteins ( acid, neutral and alkaline proteases, ácid phosphatase, ribonucleases and lipases). Lipases ( triacylglycerol hydrolases) are important enzymes in fat metabolism, catalyzing the breakdown of triacilglycerols to free fatty acids and glycerol. Owing to the very low solubility of ther natural substrats, this hydrol...
Isolation, characterization and expression of genes encoding enzymes with lipas...
Choupina, Altino; Gonzalez, Francisco J.; Fermiñán, E.; Burguillo, F.J.; Domínguez, A.Yarrowia lipolytica is a dimophic yeast with very high secretory capacities and able to grow in alkanes and fatty acids as the sole carbon source. Using PCR, we have isolated two DNA fragments that after sequencing show homology with fungal lipases. By genomic DNA subcloning, we have isolated a gene nominated YlLIP1 located on Y. lipolytica chromosome IV. The gene presents an ORF of 1458 bp which encodes a puta...
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