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1,3-Propanediol production in a two-step process fermentation from renewable fe...
Mendes, Filipa Soares; González-Pajuelo, Maria; Cordier, Hélène; François, Jean M.; Vasconcelos, IsabelIn this work, the production of 1,3-propanediol from glucose and molasses was studied in a two-step process using two recombinant microorganisms. The first step of the process is the conversion of glucose or other sugar into glycerol by the metabolic engineered Saccharomyces cerevisiae strain HC42 adapted to high (>200 g l−1) glucose concentrations. The second step, carried out in the same bioreactor, was perfo...
Antimicrobial resistance patterns inEnterobacteriaceae isolated froman urbanwas...
Silva, Miguel Ferreira da; Vaz-Moreira, Ivone; Gonzalez-Pajuelo, Maria; Nunes, Olga C.; Manaia, Célia M.Over 18 months, enterobacteria were isolated from the raw (189 isolates) and treated (156 isolates) wastewater of a municipal treatment plant. The isolates were identified as members of the genera Escherichia (76%), Shigella (7%), Klebsiella (12%) and Acinetobacter (4%). Antimicrobial susceptibility phenotypes were determined using the agar diffusion method for the antibiotics amoxicillin, gentamicin, ciproflox...
Microbial conversion of glycerol to 1,3-propanediol$ephysiological comparison o...
González-Pajuelo, María; Meynial-Salles, Isabelle; Mendes, Filipa; Soucaille, Philippe; Vasconcelos, IsabelClostridium acetobutylicum is not able to grow on glycerol as the sole carbon source since it cannot reoxidize the excess of NADH generated by glycerol catabolism. Nevertheless, when the pSPD5 plasmid, carrying the NADH-consuming 1,3-propanediol pathway from C. butyricum VPI 3266, was introduced into C. acetobutylicum DG1, growth on glycerol was achieved, and 1,3-propanediol was produced. In order to compare th...
Metabolic engineering of clostridium acetobutylicum for the industrial producti...
González-Pajuelo, María; Meynial-Salles, Isabelle; Mendes, Filipa; Andrade, Jose Carlos; Vasconcelos, Isabel; Soucaille, PhilippeClostridium butyricum is to our knowledge the best natural 1,3-propanediol producer from glycerol and the only microorganism identified so far to use a coenzyme B12-independent glycerol dehydratase. However, to develop an economical process of 1,3-propanediol production, it would be necessary to improve the strain by a metabolic engineering approach. Unfortunately, no genetic tools are currently available for C...
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