Olive mill wastewater (OMW) characteristics make it a suitable resource to be used as a microbial culture media to produce value-added compounds, such as enzymes. In this work, the ability of the novel species Aspergillus ibericus to discolor OMW and produce lipase was studied. An initial screening on plates containing an OMW-based agar medium and an emulsified olive oil/rhodamine-B agar medium was employed to ...
The fish canning industry generates large volume of wastewater for which the treatment is particularly difficult due to the high content of organic matter and salts and to the significant amount of oil and grease they present. In this work, a closed jacketed batch reactor was used to study the feasibility of applying a Fenton reaction step after an activated sludge biological treatment. For this purpose and in ...
In the Mediterranean basin countries, huge amounts of olive mill wastewaters (OMW) are produced by the olive oil industry. It constitutes a serious environmental problem, nevertheless its composition turns OMW into a potential growth medium to lipolytic microorganisms. The aim of this work was to study lipase production as well as OMW degradation in fed-batch cultures of Candida cylindracea CBS 7869, Candida ru...
Tese de doutoramento em Química e Engenharia Biológica ; Mediterranean countries are known to have favorable conditions for olive oil production. The three-phase extraction technology demands the addition of hot water to the process, and olive oil, olive cake and olive mill wastewater (OMW) are produced. An approach for using this waste as a renewable resource is of greater interest. Accordingly, the present i...
The continuous system for olive oil production can be operated by three and two-phase extraction technologies, diverging in the water supplies. The three-phase extraction process has a slightly better yield, leading to less amount of olive cake but a significant production of olive mill wastewater (OMW). The aim of the present work is the OMW valorization by producing high-value compounds while degrading this w...
Olive mill wastewater (OMW) is a liquid waste that results in large amounts from the olive oil manufacturing industry. The quality and quantity of OMW constituents are dependent of many factors, such as olives type and maturity, climatic conditions and region of origin, cultivation methods, and technology used for oil extraction1. From the 3-phases centrifugation process around 1.6 cubic meters of OMW per ton o...
A microtiter plate adaptation of the well-known dinitrosalicylic acid (DNS) colorimetric method, for measurement of reducing sugars, is described. This method allows a substantial volume reduction of the reagents and a rapid analysis of a large number of samples, having an economic cost benefit and a positive impact on the environment. Therefore, it is well suited as a high-throughput technique for reducing sug...
Este resumo faz parte de: Book of abstracts of the Meeting of the Institute for Biotechnology and Bioengineering, 2, Braga, Portugal, 2010. A versão completa do livro de atas está disponível em: http://hdl.handle.net/1822/10968
Este resumo faz parte de: Book of abstracts of the Meeting of the Institute for Biotechnology and Bioengineering, 2, Braga, Portugal, 2010. A versão completa do livro de atas está disponível em: http://hdl.handle.net/1822/10968
The ability of lipolytic yeasts to grow on olive mill wastewater (OMW)-based medium and to produce high-value compounds while degrading this waste, was tested. OMW collected from three-phase olive mills from the North region of Portugal were characterized and used. OMW with COD ranging from 100 g L−1 to 200 g L−1 were supplemented with yeast extract and ammonium chloride. Studies of OMW consumption were carried...
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