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Comparative evaluation of immunofluorescent antibody and new immunoblot tests f...
Schares, G.; Basso, W.; Majzoub, M.; Rostaher, A.; Scharr, J.C.; Langenmayer, M.C.; Selmair, J.; Dubey, J.P.; Cortes, H.C.; Conraths, F.J.Besnoitia besnoiti, an economically important disease in cattle in many countries of Africa and Asia is re-emerging in Europe. Serological identification of infected cattle is important because introduction of these animals into naive herds seems to play a major role in the transmission of the parasite. We report new, simplified immunoblot-based serological tests for the detection of B. besnoiti specific antibo...
First in vitro isolation of Besnoitia besnoiti from chronically infected cattle...
Schares, G.; Basso, W.; Majzoub, M.; Cortes, H.C.E.; Rostaher, A.; Selmair, J.; Hermanns, W.; Conraths, F.J.; Gollnick, N.S.Besnoitia besnoiti was in vitro isolated during the first recorded outbreak of bovine besnoitiosis in Germany. Molecular characterization of the new isolate, named Bb-GER1, revealed almost 100% identity with other B. besnoiti isolates obtained in Portugal, Spain, Israel or South Africa, when partial sequences of the 18S ribosomal RNA gene, of the internal transcribed spacer 1 and of the 5.8S RNA gene were compa...
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