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Functional characterization of a xylose transporter in Aspergillus nidulans
Rodrigues, Fernando José dos Santos; Menino, João F.; Colabardini, Ana Cristina; Ries, Laure Nicolas Annick; Brown, Neil AndrewBACKGROUND: The production of bioethanol from lignocellulosic feedstocks will only become economically feasible when the majority of cellulosic and hemicellulosic biopolymers can be efficiently converted into bioethanol. The main component of cellulose is glucose, whereas hemicelluloses mainly consist of pentose sugars such as D-xylose and L-arabinose. The genomes of filamentous fungi such as A. nidulans encode...
Identification of metabolic pathways influenced by the G-protein coupled recept...
Souza, Wagner R. de; Morais, Enyara Rezende; Krohn, Nadia Graciele; Savoldi, Marcela; Goldman, Maria Helena S.; Rodrigues, Fernando; Caldana, CamilaHeterotrimeric G-protein-mediated signaling pathways play a pivotal role in transmembrane signaling in eukaryotes. Our main aim was to identify signaling pathways regulated by A. nidulans GprB and GprD G-protein coupled receptors (GPCRs). When these two null mutant strains were compared to the wild-type strain, the DeltagprB mutant showed an increased protein kinase A (PKA) activity while growing in glucose 1% ...
Identification of glucose transporters in Aspergillus nidulans
Reis, Thaila Fernanda dos; Menino, João F.; Pedro Bom, Vinícius Leite; Brown, Neil Andrew; Colabardini, Ana Cristina; Savoldi, Marcelao characterize the mechanisms involved in glucose transport, in the filamentous fungus Aspergillus nidulans, we have identified four glucose transporter encoding genes hxtB-E. We evaluated the ability of hxtB-E to functionally complement the Saccharomyces cerevisiae EBY.VW4000 strain that is unable to grow on glucose, fructose, mannose or galactose as single carbon source. In S. cerevisiae HxtB-E were targeted ...
Trichoderma harzianum transformant has high extracellular alkaline proteinase e...
Goldman,Maria Helena S.; Goldman,Gustavo H.The mycoparasite Trichoderma harzianum produces an alkaline proteinase that may be specifically involved in mycoparasitism. We have constructed transformant strains of this fungus that overexpress this alkaline proteinase. Some of the transformants were assessed for alkaline proteinase activity, and those with higher activity than the wild type were selected for further studies. One of these transformant strain...
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