Encontrados 13 documentos, a visualizar página 1 de 2
Análise dos fluxos metabólicos em Saccharomyces cerevisiae a partir de D-xilulo...
Cavalcanti-Montano, I. D.; Suarez, C. A. G.; Sousa Jr., R.; Giordano, R. C.; Ferreira, E. C.; Zangirolami, T. C.; Rocha, I.A viabilidade econômica da produção de etanol 2G depende da eficiente fermentação da fração hemicelulósica por S. cerevisiae. A xilulose, isômero da xilose e principal componente da hemicelulose do bagaço, pode ser convertida pela levedura em biomassa, etanol, xilitol ou outros metabólitos. A otimização da produção de etanol requer a análise do metabolismo da xilulose. Modelos metabólicos permitem efetuar simul...
Avaliação in silico e em cultivos contínuos do metabolismo de S. typhimurium
Sargo, C. R.; Zangirolami, T. C.; Giordano, R. C.; Ferreira, E. C.; Rocha, I.; Silva, A. J.Linhagens atenuadas de Salmonella têm sido investigadas para o desenvolvimento de diversos produtos biotecnológicos, como vacinas recombinantes. No entanto, protocolos para obtenção de altas densidades celulares de Salmonella spp., necessárias para viabilizar o processo de produção destes produtos, ainda não foram estabelecidos, sendo necessário para isso contornar a elevada formação de produtos tóxicos de seu ...
Using a medium of free amino acids to produce penicillin g acylase in fed-batch...
Silva,R. G.; Souza,V. R.; Nucci,E. R.; Pinotti,L. M.; Cruz,A. J. G.; Giordano,R. C.; Giordano,R. L.C.The production of penicillin G acylase (PGA, an important industrial enzyme) from a wild strain of Bacillus megaterium using a pool of free amino acids as substrate was studied in a bench-scale bioreactor. Experiments carried out in shakers showed that the substitution of casein for free amino acids in the presence of cheese whey was the culture medium that provided the highest productivity. Several cultivation...
A fuzzy logic algorithm for identification of the harvesting threshold during P...
Nucci,E. R.; Silva,R. G.; Gomes,T. C.; Giordano,R. C.; Cruz,A. J. G.Penicillin G acylase (PGA) is an important enzyme used as biocatalyst in the production of semisynthetic beta-lactam antibiotics. Many microorganisms produce this enzyme and recombinant Escherichia coli has been preferred use for industrial applications. Bacillus megaterium is one of the microorganisms that excretes this enzyme into the medium. As a consequence, separation and purification steps are simplified....
Estimation of mass transfer parameters in a Taylor-Couette-Poiseuille heterogen...
Resende,M. M.; Vieira,P. G.; Sousa Jr,R.; Giordano,R. L. C.; Giordano,R. C.A bench-scale, continuous vortex flow reactor (VFR), with a radius ratio, h, equal to 0.48 and an aspect ratio, G, equal to 11.19 was studied. This reactor may be used in the enzymatic hydrolysis of polypeptides obtained from sweet cheese whey with enzymes immobilized on agarose gel. Operational conditions were 2410 < Re q < 11793 and 30-min residence time for glycerol-water, 14% w/w, 27ºC (Re ax = 1.1) and for...
Kinetic model for whey protein hydrolysis by alcalase multipoint-immobilized on...
Sousa Jr,R.; Lopes,G. P.; Tardioli,P. W.; Giordano,R. L. C.; Almeida,P. I. F.; Giordano,R.C.Partial hydrolysis of whey proteins by enzymes immobilized on an inert support can either change or evidence functional properties of the produced peptides, thereby increasing their applications. The hydrolysis of sweet cheese whey proteins by alcalase, which is multipoint-immobilized on agarose gel, is studied here. A Michaelis-Menten model that takes into account competitive inhibition by the product was fitt...
Improving selectivity and productivity of the enzymatic synthesis of ampicillin...
Ferreira,A. L. O.; Giordano,R. L. C.; Giordano,R. C.An experimental design was applied to improve the reaction conditions for enzymatic synthesis of ampicillin from phenylglycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA), catalyzed by penicillin G acylase from E. coli immobilized on an agarose-glyoxyl derivative. The presence and magnitude of interactions between reaction variables were estimated using a 2(5) factorial design. A batch reactor was ...
A hybrid feedforward neural network model for the cephalosporin C production pr...
Silva,R.G.; Cruz,A.J.G.; Hokka,C.O.; Giordano,R.L.C.; Giordano,R.C.At present, direct on-line measurements of key bioprocess variables as biomass, substrate and product concentrations is a difficult task. Many of the available hardware sensors are either expensive or lack reliability and robustness. To overcome this problem, indirect estimation techniques have been studied during the last decade. Inference algorithms rely either on phenomenological or on empirical models. Rece...
A simplified kinetic model for the side reactions occurring during the enzymati...
Ferreira,A.L.O.; Gonçalves,L.R.B.; Giordano,R.C.; Giordano,R.L.C.This work presents a kinetic study of the side reactions of the ampicillin enzymatic synthesis, from phenylglycine methyl ester and 6-aminopenicillanic acid using penicillin G acylase immobilized on agarose. A Michaelis-Menten model with competitive inhibition was fitted to initial rates of ester and antibiotic hydrolysis, at pH 6.5 and 25ºC. Inherent kinetic parameters were estimated for low enzymatic loads, t...
Estimation of the volumetric oxygen tranfer coefficient (KLa) from the gas bala...
CRUZ,A. J. G.; SILVA,A. S.; ARAUJO,M. L. G. C.; GIORDANO,R. C.; HOKKA,C. O.This paper reports on the use of the gas balance and dynamic methods to obtain an estimate of the volumetric oxygen transfer coefficient (kLa) in a conventional reactor during the growth phase of the microorganism Cephalosporium acremonium. A new way of calculating kLa by the dynamic method employing an electrode with a slow response, is proposed. The calculated values of kLa were used in the training of a feed...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||