Encontrados 16 documentos, a visualizar página 1 de 2
Characterization of bacPPK34 a bacteriocin produced by Pediococcus pentosaceus ...
Abrams, Daniel; Barbosa, Joana; Albano, Helena; Silva, Joana; Gibbs, Paul A.; Teixeira, PaulaDifferent lactic acid bacteria were isolated during different stages in the production of “Alheiras”, a traditionally fermented sausage produced in the north of Portugal, between 2005 and 2007, in a total of 484 isolates. One of 484 isolates (K34) produced a bacteriocin, designated as bacPPK34, and was identified as a strain of Pediococcus pentosaceus by 16S rRNA sequencing. The highest bacteriocin production w...
Numeric taxonomy approaches for lytic evaluation of Salmonella specific bacteri...
Amorim, Lara R. P.; Tamames, Juan A. C.; Silva, Joana G. L.; Gibbs, Paul A.; Teixeira, Paul C.This study explored the lytic ability of bacteriophages as a future tool for reducing the Salmonella spp. loads in food animals. It investigated (a) the concept of a phage cocktail resulting from an exploratory analysis of the 13 phages which were examined, and (b) the possibility of using them in phage typing techniques for a broad range of serotypes. By using the conventional plaque assay method and cluster a...
Characterization of anti-listeria bacteriocins isolated from shellfish: Potenti...
Pinto, Ana Luísa; Fernandes, Melissa; Pinto, Cristina; Albano, Helena; Castilho, Fernanda; Teixeira, Paula; Gibbs, Paul A.This work had as main objectives to characterize two bacteriocins produced by lactic acid bacteria (LAB) previously isolated from non-fermented seafood, in order to evaluate their potential as new food protective agents. The two bacteriocinogenic isolates were identified by Polymerase Chain Reaction (PCR) using genusand species-specific primers, and confirmed by 16S rDNA sequencing, as Enterococcus faecium and ...
Application of an impedimetric technique for the detection of lytic infection o...
Amorim, Lara R. P.; Silva, Joana G. L.; Gibbs, Paul A.; Teixeira, Paula C.This study was performed to evaluate the adaption of the impedimetric method to detect the lytic infection by Salmonella-specific bacteriophages and to provide a higher selectivity to this rapid method in detecting Salmonella spp. by using specific agents. Three bacteriophages and twelve strains of Salmonella spp. were tested. Each of the twelve strains was used separately to inoculate TSB together with each on...
Growth control of listeria innocua 2030c on vacuum-packaged cold-smoked salmon ...
Tomé, Elisabetta; Gibbs, Paul A.; Teixeira, Paula C.Five bacteriocin-producing lactic acid bacteria (LAB): Enterococcus faecium ET05, Lactobacillus curvatus ET06, L. curvatus ET30, L. deldrueckii ET32 and Pediococcus acidilactici ET34, selected by their capacity for growth and producing inhibition in vitro at high salt-on-water content, low temperature and anaerobic atmosphere, conditions simulating cold-smoked fish,were inoculated onto salmon fillets, in co-cul...
In vitro tests of suitability of bacteriocin-producing lactic acid bacteria, as...
Tomé, Elisabetta; Pereira, Vera L.; Lopes, Carla I.; Gibbs, Paul A.; Teixeira, Paula C.The objectives of this study were to ascertain the in vitro potential of nine bacteriocin-producing lactic acid bacteria (LAB) strains, isolated from vacuum-packaged cold-smoked salmon (CSS), for possible use as biopreservative cultures against Listeria monocytogenes. The antilisterial activity of cultures’ supernatants was assessed at 0.5%, 3.0% and 5.0% w/v of salt, at 5ºC, 10ºC and 25ºC both in aerobic and a...
Antimicrobial effects of a microemulsion and a nanoemulsion on enteric and othe...
Teixeira, Paula C.; Leite, Gonçalo M.; Domingues, Ricardo J.; Silva, Joana; Gibbs, Paul A.; Ferreira, João PauloSome microemulsions and nanoemulsions may have antimicrobial properties and be effective anti-biofilm agents. We examined the abilities of two fine emulsions, designated BCTP and TEOP, to inactivate suspensions of vegetative cells of Salmonella spp. Escherichia coli 0157:H7 (VT-), Pseudomonas aeruginosa, Staphylococcus aureus and Listeria monocytogenes. BCTP is an O/W nanoemulsion of soybean oil and tri-n-butyl...
Listeriosis in Portugal: an existing but under reported infection
Almeida, Gonçalo N.; Gibbs, Paul A.; Hogg, Tim A.; Teixeira, Paula C.Listeriosis is a rare disease caused by the bacterium Listeria monocytogenes, the normal vehicle of which is food. The disease, which is largely confined to its risk groups of pregnant women, the elderly and immunocompromised individuals, has increased in incidence in recent years. In Portugal, listeriosis is not a notifiable infection and available data are scarce. The objective of this work was to collate the...
Anti-listerial inhibitory lactic acid bacteria isolated from commercial cold sm...
Tome, Elisabetta; Teixeira, Paula; Gibbs, Paul A.The natural microflora of cold-smoked fish at the end of shelf-life are lactic acid bacteria (LAB). Some of these display a capacity to inhibit spoilage as well as several strains of pathogenic micro-organisms, e.g. Listeria monocytogenes which is isolated frequently from cold-smoked salmon (CSS). Eight batches of sliced vacuum-packed CSS from Norway, Scotland and Spain were collected at retail. Packs were stor...
Incidence of Listeria spp. in domestic refrigerators in Portugal
Azevedo, Inês; Regalo, Mafalda; Mena, Cristina; Almeida, Gonçalo; Carneiro, Luísa; Teixeira, Paula; Hogg, Tim; Gibbs, Paul A.The main objectives of this study were to determine the incidence of Listeria spp. in Portuguese domestic refrigerators and to evaluate some of the hygienic practices in the domestic environment that might contribute to the persistence of the organisms. It was found that L. monocytogenes was present in 3 domestic refrigerators of the 86 investigated. L. grayi and L. innocua were also isolated from 4 and 1 refri...
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