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Rapid vascularization of starchâ poly(caprolactone) in vivo by outgrowth endot...
Ghanaati, Shahram; Fuchs, Sabine; Webber, M. J.; Orth, Carina; Barbeck, Mike; Gomes, Manuela E.; Reis, R. L.; Kirkpatrick, C. JamesThe successful integration of in vitro-generated tissues is dependent on adequate vascularization in vivo. Human outgrowth endothelial cells (OECs) isolated from the mononuclear cell fraction of peripheral blood represent a potent population of circulating endothelial progenitors that could provide a cell source for rapid anastomosis and scaffold vascularization. Our previous work with these cells in co-culture...
Contribution of outgrowth endothelial cells from human peripheral blood on in v...
Fuchs, Sabine; Ghanaati, Shahram; Orth, Carina; Barbeck, Mike; Kolbe, Marlen; Hofmann, Alexander; Eblenkamp, Markus; Gomes, Manuela E.; Reis, R. L.In the present study we assessed the potential of human outgrowth endothelial cells (OEC), a subpopulation within endothelial progenitor cell cultures, to support the vascularization of a complex tissue engineered construct for bone. OEC cultured on starch polycaprolactone fiber meshes (SPCL) in monoculture retained their endothelial functionality and responded to angiogenic stimulation by VEGF (vascular endoth...
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