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Solute transport across plant cell membranes

Conde, Artur; Martins, Viviana; Noronha, Henrique; Conde, Carlos Alberto da Silva; Fontes, N.; Gerós, H.

CanaBQ is a Journal of the Portuguese Biochemical Society (http://canalbq.spb.pt/index.html) ; The transport of solutes across cell membranes, including organic nutrients, such as sugar, osmolytes, ions or metabolic waste products, is of extreme importance in all living systems. Up to 14% of the genome of all organisms represents information for transport proteins, which reflects the importance of such process...


Membrane transport, sensing and signaling in plant adaptation to environmental ...

Conde, Artur; Chaves, M. Manuela; Gerós, H.

This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Plant and Cell Physiology following peer review. The definitive publisher-authenticated version (Conde A, Chaves MM, Gerós H. 2011. Membrane transport, sensing and signaling in plant adaptation to environmental stress. Plant Cell Physiol. in press. doi: 10.1093/pcp/pcr107) is available online at: http://pcp.oxfordjournals....


Grape berry vacuole : a complex and heterogeneous membrane system specialized i...

Fontes, N.; Gerós, H.; Delrot, Serge

Vacuoles fulfill highly specialized functions depending on cell type and tissue and plant developmental stage. This complex and dynamic organelle is the main reservoir of grape berry cells, playing a major role during fruit development and ripening. Berry development is accompanied by modifications in size, composition, color, texture, flavor, and pathogen susceptibility, primarily because of changes in vacuola...


New observations on the integrity, structure and physiology of flesh cells from...

Fontes, N.; Côrte-Real, Manuela; Gerós, H.

The physiological/structural status of the soft ripened berry is still a matter of debate. In this paper isolated mesocarp cells from ripened berries of both wine and table varieties were studied by bright-field, fluorescence and confocal microscopy and flow cytometry to highlight the organization of berry flesh cell, function and viability. Flow cytometry analysis confirmed that protoplasting from grape berry ...


The effect of heat and salicylic acid on the DAHPS expression and activity in g...

Noronha, Henrique; Teixeira, António; Gerós, H.

High temperatures affect grape berry set and development, alter the normal sugar content of the fruit, and reduce the accumulation of anthocyanins. Also, peaks of high temperature may stop the ripening progress. Despite its impact in fruit and wine quality, the mechanisms contributing to the reduction of anthocyanin levels under high temperature are still elusive. Some studies indicate that this reduction is ca...


Mannitol transport and oxidation are synchronized in Olea europaea under salt a...

Conde, Artur; Silva, Paulo; Agasse, A.; Conde, Carlos; Gerós, H.

The intracellular accumulation of organic compatible solutes acting as osmoprotectants, such as polyols, is an essential response mechanism of several plants to drought and salinity. In Olea europaea a mannitol transport system (OeMaT1) has been previously characterised as a key player in plant response to salinity, and here, heterotrophic sink models, such as olive suspension-cultured cells and fruit tissues, ...


Copper impacts on grape berry cells : uptake and detoxification

Martins, Viviana; Hanana, Mohsen; Blumwald, Eduardo; Gerós, H.

Copper has been extensively used as the active principle of fungicides, since the late 1800s when the “Bordeaux mixture” was developed and its spectacular efficiency proved against fungal pathogens such as downy mildew, which is a large threat to winegrowers. Although initially it seemed to improve plant growth in unproductive lands, repeated use of copper-based fungicides has led to the accumulation of large c...


Um tributo ao manitol em Olea europaea

Conde, Artur; Gerós, H.

A elevada salinidade e a secura são factores ambientais que afectam negativamente o crescimento das plantas e, consequentemente, a sua produtividade. O excesso de sal e o deficit hídrico podem causar citotoxicidade iónica, stresse hiperosmótico e oxidativo e desequilíbrios nutricionais. Contudo, as plantas de habitats salinos ou secos, como a oliveira (Olea europaea), exibem diversas adaptações morfológicas, fi...


In vitro cultures of grape tissues : new possibilities to study grape berry phy...

Breia, Richard; Serôdio, J.; Gerós, H.; Cunha, Ana

Grape berries suffer important morphological, biochemical and physiological changes during its development and maturation. It is known that photoassimilates translocated from leaves serve as the major source of carbon and energy to support fruit needs, but recent findings revealed that, at least in the green phase, grape berries show high photosynthetic activity especially in the exocarp. The contribution of fr...


Vacuole-mitochondrial crosstalk during apoptosis induced by acetic acid in yeast

Chaves, S. R.; Coutinho, Clara Pereira; Marques, Carolina; Rodrigues, Andreia; Salin, Bénédict; Alves, Sara Cristina Sequeira; Silva, Rui; Gerós, H.


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia