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The immobilization of polyethylene imine nano and microspheres on glass using h...
Shimanovich, Ulyana; Paulo, Artur Cavaco; Gedanken, AharonThe present article describes the creation and immobilization of Polyethylene imine (PEI) capsules on a glass surface. The synthesis and deposition were accomplished by short-time (3 min) one-step reaction. The preparation and immobilization of PEI spheres, was carried out using an environmental friendly method, the ultrasonic emulsification. The ultrasonic technique enables to control size and fulfillment of t...
Proteinaceous microspheres for targeted RNA delivery prepared by an ultrasonic ...
Shimanovich, Ulyana; Eliaz, Dror; Zigdon, Sally; Volkov, Vadim; Aizer, Adva; Paulo, Artur Cavaco; Michaeli, S.; Shav-Tal, Yaron; Gedanken, AharonIn the present work we used sonochemically prepared proteinaceous BSA spheres as a novel RNA-delivery system. The preparation of RNA-loaded BSA spheres was accomplished using an environmental friendly method termed the “ultrasonic emulsification method”. It was demonstrated that ultrasonic waves do not cause the RNA chains to degrade and the RNA molecules remain untouched. The BSA–RNA complex was successfully i...
Fragrance release profile from sonochemically prepared protein microsphere cont...
Tzhayik, O.; Paulo, Artur Cavaco; Gedanken, AharonProtein microspheres have been prepared by sonicating a mixture of pure fragrant oil (amyl acetate (AA)) with an aqueous protein (bovine serum albumin) solution. The prepared protein spheres are nano- to micrometer sized with an encapsulation efficiency of approx. 97% for the AA present on the surface and inside the BSA capsule. Containers were found stable for more than 6 months when stored sealed at 4 °C and ...
Sonochemical coating of cotton and polyester fabrics with “Antibacterial” BSA a...
Nitzan, Yeshayahu; Paulo, Artur Cavaco; Shimanovich, Ulyana; Gedanken, AharonA novel antibacterial coating for cotton and polyester fabrics has been developed by using drug-loaded proteinaceous microspheres made of bovine serum albumin and casein proteins. The microbubbles were created and anchored onto the fabrics (see figure) in a one-step reaction that lasts 3 min. The sonochemically produced “antibacterial fabrics” have been characterized. The efficiency of the sonochemical process ...
Releasing dye encapsulated in proteinaceous microspheres on conductive fabrics ...
Shimanovich, Ulyana; Perelshtein, Ilana; Paulo, Artur Cavaco; Gedanken, AharonThe current paper reports on the relase properties of conductive fabrics coated with proteinaceous microspheres containing a dye. The release of the dye was achieved by passing an electric current through the fabric. The conductivity of the polyester fibers resulted from nanosilver (Ag NPs) coated on the surface of these fibers. Both types of coatings (nanosilver coating and the coating of the proteinaceous mic...
Insights on the mechanism of formation of protein microspheres in a biphasic sy...
Silva, Raquel; Ferreira, Helena; Azoia, Nuno G.; Shimanovich, Ulyana; Freddi, G.; Gedanken, Aharon; Paulo, Artur CavacoMicrospheres of bovine serum albumin (BSA) and silk fibroin are produced by applying ultrasound in a biphasic system consisting of an aqueous protein solution and an organic solvent. The protein microspheres are dispersed in an aqueous media where the protein remains at the interface covering the organic solvent. This only occurs when high shear forces are applied that induce changes to force the protein to the...
Encapsulation of RNA molecules in BSA microspheres and internalization into Try...
Shimanovich, Ulyana; Tkacz, Itai Dov; Eliaz, Dror; Paulo, Artur Cavaco; Michaeli, S.; Gedanken, AharonRNA was encapsulated in bovine serum albumin (BSA) microspheres using a one-step sonochemical process from an water–oil solvent biphasic system. Confocal microcoscopy and fluorescence-activated cell sorting indicate that a CY3-RNA (RNA labeled with red fluorescent indocarbocyanine Cy3 dye) sphere is encapsulated in the BSA outer sphere. The diameter of the sphere depends on the number of nucleotides of the RNA,...
Microspheres of mixed proteins
Angel, U.; Matas, D.; Michaeli, S.; Paulo, Artur Cavaco; Gedanken, AharonThis paper describes the synthesis of mixed proteinaceous microspheres (MPMs) by the sonochemical method. The current fundamental research follows the research of Suslick and co-workers who have developed a method by which high-intensity ultrasound is used to make aqueous suspensions of proteinaceous microcapsules filled with water-insoluble liquids.1 By using high-intensity ultrasound, we have synthesized micr...
Attaching different kinds of proteinaceous nanospheres to a variety of fabrics ...
Angel, U.; Silva, Carla Manuela; Paulo, Artur Cavaco; Gedanken, AharonThe application of a rapid, non-destructive, cost-effective technique such as ultrasonic emulsification for the coating of different textiles was explored. The technical benefits for this research were the generation of multifunctional materials and their combinations through environmentally friendly processing technologies. We have shown for the first time that ultrasonic waves can be used to coat proteinaceou...
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